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1.发明申请NOVEL BETA-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR ENHANCING ENZYME ACTIVITY 失效新型β-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE，其编码的基因和用于增强酶活性的方法公开(公告)号：US20120282659A1
公开(公告)日：2012-11-08
申请号：US13455770
申请日：2012-04-25
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译： 本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。
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2.发明授权β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity 失效β-半乳糖苷-α2,6-唾液酸转移酶，其编码基因，以及提高酶活性的方法公开(公告)号：US08187853B2
公开(公告)日：2012-05-29
申请号：US12529496
申请日：2008-03-03
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
IPC分类号： C12N9/10
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译： 本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。
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3.发明申请Novel Beta-Galactoside-Alpha 2,3-Sialyltransferase, a Gene Encoding Thereof, and a Method for Producing Thereof 失效新型β-半乳糖苷-α-2,3-唾液酸转移酶及其编码基因及其生产方法公开(公告)号：US20100291631A1
公开(公告)日：2010-11-18
申请号：US11918328
申请日：2006-03-31
CPC分类号： C12P19/26 , C12N9/1081 , C12Y204/99004
摘要： The present invention provides a novel β-galactoside-α2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.
摘要翻译： 本发明提供了一种新型的半乳糖苷-α2,3-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物，以及使用这种微生物生产唾液酸转移酶的方法。 本发明还提供了携带编码唾液酸转移酶的核酸和载体转化的宿主细胞的载体以及重组α-半乳糖苷-α2,3-唾液酸转移酶的制备方法。 本发明还提供了使用本发明的唾液酸转移酶制备唾液酸糖链的方法。
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4.发明申请Novel Beta-Galactoside Alpha 2,6-Sialyltransferase, Gene Coding For The Transferase And Process For Producing The Same 失效新型β-半乳糖苷α2,6-唾液酸转移酶，转移酶的基因编码及其生产方法公开(公告)号：US20090291471A1
公开(公告)日：2009-11-26
申请号：US12225148
申请日：2006-08-10
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译： 本发明提供了具有高生产力和/或高活性的新型β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用该载体转化的宿主细胞，以及用于产生重组β-半乳糖苷-α2,6-唾液酸转移酶的方法。
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公开(公告)号：US08343727B2
公开(公告)日：2013-01-01
申请号：US12675524
申请日：2008-08-28
申请人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
发明人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
IPC分类号： G01N33/53
CPC分类号： C07K17/06 , C07K14/375 , C07K2319/20 , G01N2333/36
摘要： The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly.The method of the present invention for binding a protein to a carrier comprises: preparing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds.
摘要翻译： 本发明提供了将蛋白质与载体结合的方法，使得蛋白质在其功能上不受损害，但可以比直接结合时更有效地起作用。 将蛋白质与载体结合的本发明的方法包括：制备生物素结合的载体; 制备具有与蛋白糊精结合的蛋白质的融合蛋白; 并通过蛋白酶 - 生物素键将蛋白质与载体结合。
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6.发明申请公开(公告)号：US20100311076A1
公开(公告)日：2010-12-09
申请号：US12675524
申请日：2008-08-28
申请人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
发明人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
CPC分类号： C07K17/06 , C07K14/375 , C07K2319/20 , G01N2333/36
摘要： The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly.The method of the present invention for binding a protein to a carrier comprises: preparing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds.
摘要翻译： 本发明提供了将蛋白质与载体结合的方法，使得蛋白质在其功能上不受损害，但可以比直接结合时更有效地起作用。 将蛋白质与载体结合的本发明的方法包括：制备生物素结合的载体; 制备具有与蛋白糊精结合的蛋白质的融合蛋白; 并通过蛋白酶 - 生物素键将蛋白质与载体结合。
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公开(公告)号：US07713722B2
公开(公告)日：2010-05-11
申请号：US11665568
申请日：2005-09-30
CPC分类号： C12N9/1048
摘要： The present invention provides an inexpensive and simple method which allows efficient glycosylation with glycosyltransferases derived from microorganisms of the Vibrionaceae family when compared to conventional enzymatic reaction systems.According to the method of the present invention, glycosyltransferases derived from microorganisms of the Vibrionaceae, such as β-galactoside-α2,6-sialyltransferase derived from Photobacterium damselae, β-galactoside-α2,3-sialyltransferase derived from Photobacterium phosphoreum and β-galactoside-α2,3-sialyltransferase derived from Vibrio sp., enhance their enzymatic activity when an appropriate amount of NaCl is added to their enzyme reaction systems.
摘要翻译： 本发明提供了一种便宜且简单的方法，当与常规的酶反应体系相比时，可以使用衍生自Vibrionaceae家族的微生物的糖基转移酶进行有效的糖基化。 根据本发明的方法，衍生自Vibrionaceae微生物的糖基转移酶，例如衍生自Photobacterium damselae的衍生物 - 半乳糖苷-α2,6-唾液酸转移酶，衍生自Photobacterium phospulaum的β-半乳糖苷-α2,3-唾液酸转移酶和＆bgr 来自弧菌属的β-半乳糖苷-α2,3-唾液酸转移酶，当向其酶反应体系中加入适量的NaCl时，增强其酶活性。
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8.发明授权β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity 失效β-半乳糖苷-α2,6-唾液酸转移酶，其编码基因，以及提高酶活性的方法公开(公告)号：US08372617B2
公开(公告)日：2013-02-12
申请号：US13455770
申请日：2012-04-25
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
IPC分类号： C12N9/10
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译： 本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。
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9.发明授权β-Galactoside-α2, 6-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效- 糖苷-α2,6-唾液酸转移酶，其编码基因及其制备方法公开(公告)号：US08187838B2
公开(公告)日：2012-05-29
申请号：US12789167
申请日：2010-05-27
IPC分类号： C12P21/02
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译： 本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。
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公开(公告)号：US20090087894A1
公开(公告)日：2009-04-02
申请号：US11665568
申请日：2005-09-30
IPC分类号： C12N9/10
CPC分类号： C12N9/1048
摘要： The present invention provides an inexpensive and simple method which allows efficient glycosylation with glycosyltransferases derived from microorganisms of the Vibrionaceae family when compared to conventional enzymatic reaction systems.According to the method of the present invention, glycosyltransferases derived from microorganisms of the Vibrionaceae, such as β-galactoside-α2,6-sialyltransferase derived from Photobacterium damselae, β-galactoside-α2,3-sialyltransferase derived from Photobacterium phosphoreum and β-galactoside-α2,3-sialyltransferase derived from Vibrio sp., enhance their enzymatic activity when an appropriate amount of NaCl is added to their enzyme reaction systems.
摘要翻译： 本发明提供了一种便宜且简单的方法，当与常规的酶反应体系相比时，可以使用衍生自Vibrionaceae家族的微生物的糖基转移酶进行有效的糖基化。 根据本发明的方法，衍生自Vibrionaceae的微生物的糖基转移酶，例如源自斑马鱼的β-半乳糖苷-α2,6-唾液酸转移酶，衍生自Photobacterium磷光体的β-半乳糖苷-α2,3-唾液酸转移酶和β-半乳糖苷 来自弧菌属的α2,3唾液酸转移酶，当将适量的NaCl加入其酶反应体系时，增强其酶活性。
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