利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

39 条记录 (耗时 0.036 秒)

    Novel Beta-Galactoside-Alpha 2,3-Sialyltransferase, a Gene Encoding Thereof, and a Method for Producing Thereof
    3.
    发明申请
    Novel Beta-Galactoside-Alpha 2,3-Sialyltransferase, a Gene Encoding Thereof, and a Method for Producing Thereof 失效
    新型β-半乳糖苷-α-2,3-唾液酸转移酶及其编码基因及其生产方法

    公开(公告)号：US20100291631A1

    公开(公告)日：2010-11-18

    申请号：US11918328

    申请日：2006-03-31

    申请人： Takeshi Yamamoto ,  Hiroshi Tsukamoto ,  Yoshimitsu Takakura

    发明人： Takeshi Yamamoto ,  Hiroshi Tsukamoto ,  Yoshimitsu Takakura

    IPC分类号： C12P19/44 ,  C12N9/10 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12N1/20

    CPC分类号： C12P19/26 ,  C12N9/1081 ,  C12Y204/99004

    摘要： The present invention provides a novel β-galactoside-α2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.

    摘要翻译： 本发明提供了一种新型的半乳糖苷-α2,3-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物，以及使用这种微生物生产唾液酸转移酶的方法。 本发明还提供了携带编码唾液酸转移酶的核酸和载体转化的宿主细胞的载体以及重组α-半乳糖苷-α2,3-唾液酸转移酶的制备方法。 本发明还提供了使用本发明的唾液酸转移酶制备唾液酸糖链的方法。

    β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for producing thereof
    5.
    发明授权
    β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效
    β-半乳糖苷-α2,6-唾液酸转移酶及其制备方法

    公开(公告)号：US07993875B2

    公开(公告)日：2011-08-09

    申请号：US12225148

    申请日：2006-08-10

    申请人： Hiroshi Tsukamoto ,  Toshiki Mine ,  Yoshimitsu Takakura ,  Takeshi Yamamoto

    发明人： Hiroshi Tsukamoto ,  Toshiki Mine ,  Yoshimitsu Takakura ,  Takeshi Yamamoto

    IPC分类号： C12P21/02

    CPC分类号： C12N9/1081 ,  C07H21/04

    摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.

    摘要翻译： 本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物。 本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

    β-galactoside-α2,3-sialyltransferase, a gene encoding thereof, and a method for producing thereof
    7.
    发明授权
    β-galactoside-α2,3-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效
    β-半乳糖苷-α2,3-唾液酸转移酶及其制备方法

    公开(公告)号：US08030043B2

    公开(公告)日：2011-10-04

    申请号：US11918328

    申请日：2006-03-31

    申请人： Takeshi Yamamoto ,  Hiroshi Tsukamoto ,  Yoshimitsu Takakura

    发明人： Takeshi Yamamoto ,  Hiroshi Tsukamoto ,  Yoshimitsu Takakura

    IPC分类号： C12N9/10 ,  C12N9/00 ,  C12N15/00 ,  C12N1/20 ,  C12P21/06 ,  C12P19/34 ,  C12P19/44 ,  C07H21/04

    CPC分类号： C12P19/26 ,  C12N9/1081 ,  C12Y204/99004

    摘要： The present invention provides a novel β-galactoside-α2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.

    摘要翻译： 本发明提供了一种新型的半乳糖苷-α2,3-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物，以及使用这种微生物生产唾液酸转移酶的方法。 本发明还提供了携带编码唾液酸转移酶的核酸和载体转化的宿主细胞的载体以及重组α-半乳糖苷-α2,3-唾液酸转移酶的制备方法。 本发明还提供了使用本发明的唾液酸转移酶制备唾液酸糖链的方法。

    Method for improving enzymatic activity of glycosyltransferases
    10.
    发明授权
    Method for improving enzymatic activity of glycosyltransferases 有权
    改善糖基转移酶酶活性的方法

    公开(公告)号：US07713722B2

    公开(公告)日：2010-05-11

    申请号：US11665568

    申请日：2005-09-30

    申请人： Hiroshi Tsukamoto ,  Takeshi Yamamoto ,  Yoshimitsu Takakura

    发明人： Hiroshi Tsukamoto ,  Takeshi Yamamoto ,  Yoshimitsu Takakura

    IPC分类号： C12N9/10 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/1048

    摘要： The present invention provides an inexpensive and simple method which allows efficient glycosylation with glycosyltransferases derived from microorganisms of the Vibrionaceae family when compared to conventional enzymatic reaction systems.According to the method of the present invention, glycosyltransferases derived from microorganisms of the Vibrionaceae, such as β-galactoside-α2,6-sialyltransferase derived from Photobacterium damselae, β-galactoside-α2,3-sialyltransferase derived from Photobacterium phosphoreum and β-galactoside-α2,3-sialyltransferase derived from Vibrio sp., enhance their enzymatic activity when an appropriate amount of NaCl is added to their enzyme reaction systems.

    摘要翻译： 本发明提供了一种便宜且简单的方法，当与常规的酶反应体系相比时，可以使用衍生自Vibrionaceae家族的微生物的糖基转移酶进行有效的糖基化。 根据本发明的方法，衍生自Vibrionaceae微生物的糖基转移酶，例如衍生自Photobacterium damselae的衍生物 - 半乳糖苷-α2,6-唾液酸转移酶，衍生自Photobacterium phospulaum的β-半乳糖苷-α2,3-唾液酸转移酶和＆bgr 来自弧菌属的β-半乳糖苷-α2,3-唾液酸转移酶，当向其酶反应体系中加入适量的NaCl时，增强其酶活性。