- 专利标题: Protocols for making hepatocytes from embryonic stem cells
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申请号: US12277136申请日: 2008-11-24
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公开(公告)号: US20090136955A1公开(公告)日: 2009-05-28
- 发明人: Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
- 申请人: Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12Q1/02
摘要:
This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
公开/授权文献
- US08546139B2 Protocols for making hepatocytes from embryonic stem cells 公开/授权日:2013-10-01