-
公开(公告)号：US07473555B2
公开(公告)日：2009-01-06
申请号：US10810311
申请日：2004-03-26
申请人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。
-
公开(公告)号：US08546139B2
公开(公告)日：2013-10-01
申请号：US12277136
申请日：2008-11-24
申请人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
-
公开(公告)号：US20090136955A1
公开(公告)日：2009-05-28
申请号：US12277136
申请日：2008-11-24
申请人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
-
公开(公告)号：US20050037493A1
公开(公告)日：2005-02-17
申请号：US10810311
申请日：2004-03-26
申请人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。
-
5.发明授权公开(公告)号：US07282366B2
公开(公告)日：2007-10-16
申请号：US10087142
申请日：2002-03-01
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/148 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2503/02 , C12N2506/02 , C12N2533/92
摘要： It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
摘要翻译： 已经发现，当在肝细胞分化剂存在下培养多能干细胞时，衍生出具有非常高比例的具有肝细胞表型特征的细胞的细胞群。 在一个实例中，允许人胚胎干细胞形成胚状体，然后与分选剂正丁酸酯组合，任选地补充成熟因子。 在另一个实例中，在无饲养细胞培养物中将正丁酸加入到人胚胎干细胞中。 无论哪种方式，获得了显着均匀的细胞群，其由具有肝细胞形态特征的细胞占主导，表达肝细胞特征的表达标志物，并具有对肝功能重要的酶和生物合成活性。 由于干细胞在培养物中容易增殖，所以该系统为各种应用提供了丰富的肝细胞谱系来源，如药物筛选，并在临床治疗的背景下补充肝功能。
-
公开(公告)号：US07256042B2
公开(公告)日：2007-08-14
申请号：US10001267
申请日：2001-10-31
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/148 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
摘要翻译： 已经发现，当在肝细胞分化剂存在下培养多能干细胞时，衍生出具有非常高比例的具有肝细胞表型特征的细胞的细胞群。 在一个实例中，允许人胚胎干细胞形成胚状体，然后与分选剂正丁酸酯组合，任选地补充成熟因子。 在另一个实例中，在无饲养细胞培养物中将正丁酸加入到人胚胎干细胞中。 无论哪种方式，获得了显着均匀的细胞群，其由具有肝细胞形态特征的细胞占主导，表达肝细胞特征的表达标志物，并具有对肝功能重要的酶和生物合成活性。 由于干细胞在培养物中容易增殖，所以该系统为各种应用提供了丰富的肝细胞谱系来源，如药物筛选，并在临床治疗的背景下补充肝功能。
-
公开(公告)号：US06506574B1
公开(公告)日：2003-01-14
申请号：US09872182
申请日：2001-05-31
IPC分类号： C12Q142
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/148 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2503/02 , C12N2506/02 , C12N2533/90
摘要： It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
-
公开(公告)号：US06458589B1
公开(公告)日：2002-10-01
申请号：US09718308
申请日：2000-11-20
IPC分类号： C12N508
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/148 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2503/02 , C12N2506/02 , C12N2533/90
摘要： It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
摘要翻译： 已经发现，当在肝细胞分化剂存在下培养多能干细胞时，衍生出具有非常高比例的具有肝细胞表型特征的细胞的细胞群。 在一个实例中，允许人胚胎干细胞形成胚状体，然后与分选剂正丁酸酯组合，任选地补充成熟因子。 在另一个实例中，在无饲养细胞培养物中将正丁酸加入到人胚胎干细胞中。 无论哪种方式，获得了显着均匀的细胞群，其由具有肝细胞形态特征的细胞占主导，表达肝细胞特征的表达标志物，并具有对肝功能重要的酶和生物合成活性。 由于干细胞在培养物中容易增殖，所以该系统为各种应用提供了丰富的肝细胞谱系来源，如药物筛选，并在临床治疗的背景下补充肝功能。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.039 秒)