Feeder-free culture method for embryonic stem cells or primate primordial stem cells
    2.
    发明授权
    Feeder-free culture method for embryonic stem cells or primate primordial stem cells 有权
    用于胚胎干细胞或灵长类动物原始干细胞的无饲养培养方法

    公开(公告)号:US07413902B2

    公开(公告)日:2008-08-19

    申请号:US10330873

    申请日:2002-12-24

    IPC分类号: C12N5/02

    摘要: Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt.

    摘要翻译: 描述了用于培养灵长类衍生的原始干细胞的方法和材料。 在一个实施方案中,提供了用于生长基本上未分化状态的灵长类来源的原始干细胞的细胞培养基,其包括低渗透压,有效支持灵长类来源的原始干细胞的生长的低内毒素碱性培养基。 基本培养基与有效支持灵长类衍生的原始干细胞的生长和选自饲养细胞的底物和源自饲养细胞的细胞外基质组分的营养血清组合。 培养基还包括非必需氨基酸,抗氧化剂和选自核苷和丙酮酸盐的第一生长因子。

    Tolerizing allografts of pluripotent stem cells

    公开(公告)号:US20060002906A1

    公开(公告)日:2006-01-05

    申请号:US11219477

    申请日:2005-09-02

    IPC分类号: A61K48/00 C12N5/08

    摘要: This disclosure provides a system for overcoming HLA mismatch between an allograft derived from stem cells, and a patient being treated for tissue regeneration. A state of specific immune tolerance is induced in the patient, by administering a population of tolerizing cells derived from the stem cells. This allows the patient to accept an allograft of differentiated cells derived from the same source. This invention is important because it allows a single line of stem cells to act as a universal donor source for tissue regeneration in any patient, regardless of tissue type.

    Treatment for wounds
    7.
    发明授权
    Treatment for wounds 有权
    治疗伤口

    公开(公告)号:US07262174B2

    公开(公告)日:2007-08-28

    申请号:US10143536

    申请日:2002-05-09

    IPC分类号: A61K48/00

    摘要: It has been discovered that increasing telomerase activity in cells surrounding a wound has a variety of effects that enhance wound healing. Replication capacity is enhanced, and the mobility of the epithelial cells can be increased by 3-fold or more. Some aspects of the invention relate to agents that increase telomerase activity in cells at the site of the wound, promoting cells to move to the site and restore an epithelial layer and the underlying stratum. Other aspects of the invention relate to compositions comprising epithelial cells in which telomerase activity has been increased, useful as grafts for the treatment of wounds.

    摘要翻译: 已经发现,在伤口周围的细胞中增加端粒酶活性具有增强伤口愈合的各种效果。 复制能力增强,上皮细胞的迁移率可以提高3倍以上。 本发明的一些方面涉及在伤口部位的细胞中增加端粒酶活性的试剂,促进细胞移动到位点并恢复上皮层和下层。 本发明的其它方面涉及包含其中端粒酶活性增加的上皮细胞的组合物,可用作治疗伤口的移植物。

    Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
    9.
    发明授权
    Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture 有权
    用于无饲养细胞培养的灵长类动物原始干细胞生长的方法和材料

    公开(公告)号:US06800480B1

    公开(公告)日:2004-10-05

    申请号:US09530346

    申请日:2000-08-29

    IPC分类号: C12N500

    摘要: Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.

    摘要翻译: 描述了用于培养灵长类衍生的原始干细胞的方法和材料。 在一个实施方案中,提供了用于生长基本上未分化状态的灵长类来源的原始干细胞的细胞培养基,其包括低渗透压,有效支持灵长类来源的原始干细胞的生长的低内毒素碱性培养基。 基本培养基与有效支持灵长类衍生的原始干细胞的生长的营养血清和选自饲养细胞和源自饲养细胞的细胞外基质组分的底物组合。 该培养基还包括非必需氨基酸,抗氧化剂和选自核苷和丙酮酸盐的第一生长因子。