公开(公告)号：US07473555B2

公开(公告)日：2009-01-06

申请号：US10810311

申请日：2004-03-26

申请人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。

公开(公告)号：US07413902B2

公开(公告)日：2008-08-19

申请号：US10330873

申请日：2002-12-24

申请人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

发明人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

IPC分类号： C12N5/02

CPC分类号： C12N5/0606 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2510/00

摘要： Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt.

摘要翻译： 描述了用于培养灵长类衍生的原始干细胞的方法和材料。 在一个实施方案中，提供了用于生长基本上未分化状态的灵长类来源的原始干细胞的细胞培养基，其包括低渗透压，有效支持灵长类来源的原始干细胞的生长的低内毒素碱性培养基。 基本培养基与有效支持灵长类衍生的原始干细胞的生长和选自饲养细胞的底物和源自饲养细胞的细胞外基质组分的营养血清组合。 培养基还包括非必需氨基酸，抗氧化剂和选自核苷和丙酮酸盐的第一生长因子。

公开(公告)号：US20060002906A1

公开(公告)日：2006-01-05

申请号：US11219477

申请日：2005-09-02

申请人： Choy-Pik Chiu ,  Robert Kay

发明人： Choy-Pik Chiu ,  Robert Kay

IPC分类号： A61K48/00 ,  C12N5/08

CPC分类号： A61K39/001 ,  A61K35/12 ,  A61K2035/122 ,  C12N2506/02

摘要： This disclosure provides a system for overcoming HLA mismatch between an allograft derived from stem cells, and a patient being treated for tissue regeneration. A state of specific immune tolerance is induced in the patient, by administering a population of tolerizing cells derived from the stem cells. This allows the patient to accept an allograft of differentiated cells derived from the same source. This invention is important because it allows a single line of stem cells to act as a universal donor source for tissue regeneration in any patient, regardless of tissue type.

公开(公告)号：US08546139B2

公开(公告)日：2013-10-01

申请号：US12277136

申请日：2008-11-24

申请人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12N5/00 ,  C12N5/10 ,  C12N5/071 ,  A61K49/00

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

公开(公告)号：US20090136955A1

公开(公告)日：2009-05-28

申请号：US12277136

申请日：2008-11-24

申请人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12Q1/68 ,  C12Q1/02

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

公开(公告)号：US20060239974A1

公开(公告)日：2006-10-26

申请号：US11411394

申请日：2006-04-25

申请人： Xu-Rong Jiang ,  Choy-Pik Chiu ,  Calvin Harley

发明人： Xu-Rong Jiang ,  Choy-Pik Chiu ,  Calvin Harley

IPC分类号： A61K48/00 ,  C12N15/861

CPC分类号： A61L26/0066 ,  A61K35/12 ,  A61K35/36 ,  A61K38/45 ,  A61L15/40 ,  A61L15/44 ,  A61L27/3804 ,  A61L27/3813 ,  A61L2300/258 ,  A61L2300/412 ,  C12N5/0629 ,  C12N2510/04 ,  C12N2799/022 ,  C12N2799/027

摘要： It has been discovered that increasing telomerase activity in cells surrounding a wound promotes wound healing. Replication capacity is enhanced, and the mobility of the epithelial cells can be increased by 3-fold or more. Particular aspects of the invention described in this disclosure include the use of agents that increase telomerase activity in cells at the site of the wound, promoting cells to move to the site and restore an epithelial layer and the underlying stratum.

摘要翻译： 已经发现，在伤口附近的细胞中增加端粒酶活性促进伤口愈合。 复制能力增强，上皮细胞的迁移率可以提高3倍以上。 在本公开中描述的本发明的特定方面包括使用增加伤口部位的细胞中的端粒酶活性的试剂，促进细胞移动到位点并恢复上皮层和下层。

公开(公告)号：US07262174B2

公开(公告)日：2007-08-28

申请号：US10143536

申请日：2002-05-09

申请人： Xu-Rong Jiang ,  Choy-Pik Chiu ,  Calvin B. Harley

发明人： Xu-Rong Jiang ,  Choy-Pik Chiu ,  Calvin B. Harley

IPC分类号： A61K48/00

CPC分类号： A61L26/0066 ,  A61K35/12 ,  A61K35/36 ,  A61K38/45 ,  A61L15/40 ,  A61L15/44 ,  A61L27/3804 ,  A61L27/3813 ,  A61L2300/258 ,  A61L2300/412 ,  C12N5/0629 ,  C12N2510/04 ,  C12N2799/022 ,  C12N2799/027

摘要： It has been discovered that increasing telomerase activity in cells surrounding a wound has a variety of effects that enhance wound healing. Replication capacity is enhanced, and the mobility of the epithelial cells can be increased by 3-fold or more. Some aspects of the invention relate to agents that increase telomerase activity in cells at the site of the wound, promoting cells to move to the site and restore an epithelial layer and the underlying stratum. Other aspects of the invention relate to compositions comprising epithelial cells in which telomerase activity has been increased, useful as grafts for the treatment of wounds.

摘要翻译： 已经发现，在伤口周围的细胞中增加端粒酶活性具有增强伤口愈合的各种效果。 复制能力增强，上皮细胞的迁移率可以提高3倍以上。 本发明的一些方面涉及在伤口部位的细胞中增加端粒酶活性的试剂，促进细胞移动到位点并恢复上皮层和下层。 本发明的其它方面涉及包含其中端粒酶活性增加的上皮细胞的组合物，可用作治疗伤口的移植物。

公开(公告)号：US20050037493A1

公开(公告)日：2005-02-17

申请号：US10810311

申请日：2004-03-26

申请人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12N5/071 ,  C12N5/08

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。

公开(公告)号：US06800480B1

公开(公告)日：2004-10-05

申请号：US09530346

申请日：2000-08-29

申请人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

发明人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

IPC分类号： C12N500

CPC分类号： C12N5/0606 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2510/00

摘要： Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.

摘要翻译： 描述了用于培养灵长类衍生的原始干细胞的方法和材料。 在一个实施方案中，提供了用于生长基本上未分化状态的灵长类来源的原始干细胞的细胞培养基，其包括低渗透压，有效支持灵长类来源的原始干细胞的生长的低内毒素碱性培养基。 基本培养基与有效支持灵长类衍生的原始干细胞的生长的营养血清和选自饲养细胞和源自饲养细胞的细胞外基质组分的底物组合。 该培养基还包括非必需氨基酸，抗氧化剂和选自核苷和丙酮酸盐的第一生长因子。