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    Medium for growing human embryonic stem cells
    1.
    发明授权
    Medium for growing human embryonic stem cells 有权
    培养人类胚胎干细胞的培养基

    公开(公告)号：US07455983B2

    公开(公告)日：2008-11-25

    申请号：US10949181

    申请日：2004-09-24

    申请人： Chunhui Xu ,  Yan Li ,  Ramkumar Mandalam

    发明人： Chunhui Xu ,  Yan Li ,  Ramkumar Mandalam

    IPC分类号： G01N33/53 ,  C12N1/00 ,  C12N5/383

    CPC分类号： C12N5/0606 ,  C12N5/0619 ,  C12N5/0622 ,  C12N15/1034 ,  C12N15/1096 ,  C12N2500/25 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/26 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2533/90

    摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of mouse embryonic fibroblast feeder cells to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by defined components added to the culture environment that support rapid proliferation without differentiation. The medium contains an isotonic buffer, a blend of essential nutrients such as protein and lipids, and an effective growth factor or combination of factors that promote proliferation while inhibiting differentiation. Culturing human embryonic stem cells in fresh medium on an extracellular matrix according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

    摘要翻译： 本公开提供了一种用于培养人多能干细胞的改进系统。 传统上，多能干细胞在一层小鼠胚胎成纤维细胞饲养细胞上培养以防止它们分化。 在这里描述的系统中，饲养细胞的作用被添加到支持快速增殖而不分化的培养环境中的限定成分所取代。 培养基含有等渗缓冲液，蛋白质和脂质等必需营养素的混合物，以及促进增殖同时抑制分化的有效生长因子或因子组合。 在根据本发明的细胞外基质上的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。 这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。

    Suspension culture of human embryonic stem cells
    2.
    发明授权
    Suspension culture of human embryonic stem cells 有权
    人类胚胎干细胞的悬浮培养

    公开(公告)号：US09074181B2

    公开(公告)日：2015-07-07

    申请号：US11917993

    申请日：2006-06-20

    申请人： Ramkumar Mandalam ,  Yan Li ,  Isabelle Nadeau-Demers

    发明人： Ramkumar Mandalam ,  Yan Li ,  Isabelle Nadeau-Demers

    IPC分类号： C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/26

    摘要： This disclosure provides an improved system for culturing human embryonic stem cells. The cells are cultured in suspension so as to maximize the production capacity of the culture environment. The new culture system of this invention allows for bulk proliferation of hES cells in a more cost-effective manner, which facilitates commercial production of important products for use in human therapy.

    摘要翻译： 本公开提供了用于培养人胚胎干细胞的改进的系统。 将细胞悬浮培养，以使培养环境的生产能力最大化。 本发明的新培养系统允许以更具成本效益的方式大量增殖hES细胞，这促进了用于人类治疗的重要产物的商业生产。

    Suspension Culture of Human Embryonic Stem Cells
    4.
    发明申请
    Suspension Culture of Human Embryonic Stem Cells 有权
    人类胚胎干细胞的悬浮培养

    公开(公告)号：US20100144033A1

    公开(公告)日：2010-06-10

    申请号：US11917993

    申请日：2006-06-20

    申请人： Ramkumar Mandalam ,  Yan Li ,  Isabelle Nadeau-Demers

    发明人： Ramkumar Mandalam ,  Yan Li ,  Isabelle Nadeau-Demers

    IPC分类号： C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/26

    摘要： This disclosure provides an improved system for culturing human embryonic stem cells. The cells are cultured in suspension so as to maximize the production capacity of the culture environment. The new culture system of this invention allows for bulk proliferation of hES cells in a more cost-effective manner, which facilitates commercial production of important products for use in human therapy.

    摘要翻译： 本公开提供了用于培养人胚胎干细胞的改进的系统。 将细胞悬浮培养，以使培养环境的生产能力最大化。 本发明的新培养系统允许以更具成本效益的方式大量增殖hES细胞，这促进了用于人类治疗的重要产物的商业生产。

    Marker system for preparing and characterizing high-quality human embryonic stem cells
    9.
    发明授权
    Marker system for preparing and characterizing high-quality human embryonic stem cells 有权
    用于制备和表征高质量人类胚胎干细胞的标记系统

    公开(公告)号：US07153650B2

    公开(公告)日：2006-12-26

    申请号：US10389431

    申请日：2003-03-13

    申请人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok

    发明人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5005

    摘要： This disclosure provides a system for qualifying embryonic stem cells intended for human therapy. A large-scale sequencing project has identified important markers that are characteristic of undifferentiated pluripotent cells. Combinations of these markers can be used to validate the self-renewing capacity of ES cells, and their ability to differentiate into tissue types suitable for regenerative medicine. The marker system of this invention has been used to screen feeder cells, media additives, and culture conditions that promote proliferation of stem cells without differentiation. A culture system optimized by following these markers is suitable for rapid expansion of undifferentiated cells from existing lines, or the derivation of new lines that are equally apposite for clinical use.

    摘要翻译： 本公开提供了用于限定用于人类治疗的胚胎干细胞的系统。 大规模测序项目确定了未分化多能细胞特征的重要标志物。 这些标记的组合可以用于验证ES细胞的自我更新能力，以及它们分化成适合再生医学的组织类型的能力。 本发明的标记系统已用于筛选不分化促进干细胞增殖的饲养细胞，培养基添加剂和培养条件。 通过遵循这些标记优化的培养系统适用于从现有品系中未分化细胞的快速扩增，或适用于临床应用的新品系的衍生。