发明申请
- 专利标题: Preparation of Nucleic Acid Templates for Solid Phase Amplification
- 专利标题(中): 固相扩增核酸模板的制备
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申请号: US12085508申请日: 2006-11-24
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公开(公告)号: US20100041561A1公开(公告)日: 2010-02-18
- 发明人: Niall Anthony Gormley , Jonathan Mark Boutell , Gerado Turcatti , Colin Lloyd Barnes
- 申请人: Niall Anthony Gormley , Jonathan Mark Boutell , Gerado Turcatti , Colin Lloyd Barnes
- 优先权: GB0524069.2 20051125
- 国际申请: PCT/GB2006/004407 WO 20061124
- 主分类号: C40B20/00
- IPC分类号: C40B20/00 ; C40B50/06
摘要:
The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.
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