公开(公告)号：US20100041561A1

公开(公告)日：2010-02-18

申请号：US12085508

申请日：2006-11-24

申请人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerado Turcatti ,  Colin Lloyd Barnes

发明人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerado Turcatti ,  Colin Lloyd Barnes

IPC分类号： C40B20/00 ,  C40B50/06

CPC分类号： C12Q1/6855 ,  C12Q2565/537

摘要： The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

摘要翻译： 本发明涉及制备用于固相核酸扩增的模板构建体的方法以及在固相核酸扩增方法中使用该模板的方法。 该方法包括进行两个连接反应：（a）连接反应，其中一个或多个靶多核苷酸分子的第一末端连接到表面结合的衔接子多核苷酸分子，和（b）连接反应，其中溶液相适配器 将多核苷酸分子连接到所述靶多核苷酸分子的第二末端，以便产生连接到固体支持物上的一个或多个模板构建体。