利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

31 条记录 (耗时 0.038 秒)

    Preparation of Nucleic Acid Templates for Solid Phase Amplification
    1.
    发明申请
    Preparation of Nucleic Acid Templates for Solid Phase Amplification 有权
    固相扩增核酸模板的制备

    公开(公告)号：US20100041561A1

    公开(公告)日：2010-02-18

    申请号：US12085508

    申请日：2006-11-24

    申请人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerado Turcatti ,  Colin Lloyd Barnes

    发明人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerado Turcatti ,  Colin Lloyd Barnes

    IPC分类号： C40B20/00 ,  C40B50/06

    CPC分类号： C12Q1/6855 ,  C12Q2565/537

    摘要： The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

    摘要翻译： 本发明涉及制备用于固相核酸扩增的模板构建体的方法以及在固相核酸扩增方法中使用该模板的方法。 该方法包括进行两个连接反应：（a）连接反应，其中一个或多个靶多核苷酸分子的第一末端连接到表面结合的衔接子多核苷酸分子，和（b）连接反应，其中溶液相适配器 将多核苷酸分子连接到所述靶多核苷酸分子的第二末端，以便产生连接到固体支持物上的一个或多个模板构建体。

    Preparation of nucleic acid templates for solid phase amplification
    4.
    发明授权
    Preparation of nucleic acid templates for solid phase amplification 有权
    制备用于固相扩增的核酸模板

    公开(公告)号：US08168388B2

    公开(公告)日：2012-05-01

    申请号：US12085508

    申请日：2006-11-24

    申请人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerardo Turcatti ,  Colin Lloyd Barnes

    发明人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerardo Turcatti ,  Colin Lloyd Barnes

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6855 ,  C12Q2565/537

    摘要： The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

    摘要翻译： 本发明涉及制备用于固相核酸扩增的模板构建体的方法以及在固相核酸扩增方法中使用该模板的方法。 该方法包括进行两个连接反应：（a）连接反应，其中一个或多个靶多核苷酸分子的第一末端连接到表面结合的衔接子多核苷酸分子，和（b）连接反应，其中溶液相适配器 将多核苷酸分子连接到所述靶多核苷酸分子的第二末端，以便产生连接到固体支持物上的一个或多个模板构建体。

    METHODS FOR SEQUENCING POLYNUCLEOTIDES
    8.
    发明申请
    METHODS FOR SEQUENCING POLYNUCLEOTIDES 有权
    用于测序多核苷酸的方法

    公开(公告)号：US20120053063A1

    公开(公告)日：2012-03-01

    申请号：US13219422

    申请日：2011-08-26

    申请人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

    发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

    IPC分类号： C40B20/00

    CPC分类号： C12Q1/6874 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2565/513 ,  C12Q2565/543

    摘要： Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.

    摘要翻译： 本文提供了用于测序多核苷酸分子的方法。 该方法包括提供连接到表面的多个多核苷酸分子的步骤，其中每个多核苷酸分子的第一部分连接到该表面的第一位置，并且每个多核苷酸分子的第二部分连接到该多核苷酸分子的第二位置 表面，第一和第二位置的相对接近度与第一和第二部分配对的概率相关，分离表面上的多核苷酸分子的第一和第二部分，确定第一和第二部分的序列 多核苷酸分子，并比较相对接近度和序列以确定哪个第一和第二部分配对并确定靶多核苷酸分子的序列。

    Methods for sequencing polynucleotides
    9.
    发明授权
    Methods for sequencing polynucleotides 有权
    多核苷酸测序方法

    公开(公告)号：US09029103B2

    公开(公告)日：2015-05-12

    申请号：US13219422

    申请日：2011-08-26

    申请人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

    发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6874 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2565/513 ,  C12Q2565/543

    摘要： Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.

    摘要翻译： 本文提供了用于测序多核苷酸分子的方法。 该方法包括提供连接到表面的多个多核苷酸分子的步骤，其中每个多核苷酸分子的第一部分连接到该表面的第一位置，并且每个多核苷酸分子的第二部分连接到该多核苷酸分子的第二位置 表面，第一和第二位置的相对接近度与第一和第二部分配对的概率相关，分离表面上的多核苷酸分子的第一和第二部分，确定第一和第二部分的序列 多核苷酸分子，并比较相对接近度和序列以确定哪个第一和第二部分配对并确定靶多核苷酸分子的序列。