发明申请
US20100136613A1 PRESSURE-ASSISTED MOLECULAR RECOVERY (PAMR) OF BIOMOLECULES, PRESSURE-ASSISTED ANTIGEN RETRIEVAL (PAAR), AND PRESSURE-ASSISTED TISSUE HISTOLOGY (PATH)
有权
生物分子的压力辅助分子恢复（PAMR），压力辅助抗体检索（PAAR）和压力协助组织学（PATH）
- 专利标题： PRESSURE-ASSISTED MOLECULAR RECOVERY (PAMR) OF BIOMOLECULES, PRESSURE-ASSISTED ANTIGEN RETRIEVAL (PAAR), AND PRESSURE-ASSISTED TISSUE HISTOLOGY (PATH)
- 专利标题（中）： 生物分子的压力辅助分子恢复（PAMR），压力辅助抗体检索（PAAR）和压力协助组织学（PATH）
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申请号： US12630746申请日： 2009-12-03
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公开(公告)号： US20100136613A1公开(公告)日： 2010-06-03
- 发明人: Timothy J. O'Leary , Jeffrey T. Mason , Carol B. Fowler , Robert E. Cunningham
- 申请人： Timothy J. O'Leary , Jeffrey T. Mason , Carol B. Fowler , Robert E. Cunningham
- 专利权人： The Government of the United States of America as represented by the Department of Veterans Affairs,The Government of the United States of America as represented by the Secretary of Defense,on behalf of the Armed Forces Instutite of Pathology,American Registry of Pathology
- 当前专利权人： The Government of the United States of America as represented by the Department of Veterans Affairs,The Government of the United States of America as represented by the Secretary of Defense,on behalf of the Armed Forces Instutite of Pathology,American Registry of Pathology
- 主分类号： G01N1/28
- IPC分类号： G01N1/28
摘要：
A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.
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