发明申请
US20110052551A1 CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF USE THEREFOR
审中-公开
CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF THEREFOR
- 专利标题: CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF USE THEREFOR
- 专利标题(中): CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF THEREFOR
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申请号: US12919648申请日: 2009-02-26
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公开(公告)号: US20110052551A1公开(公告)日: 2011-03-03
- 发明人: Michael Patrick Flaherty , Buddhadeb Dawn , Roberto Bolli
- 申请人: Michael Patrick Flaherty , Buddhadeb Dawn , Roberto Bolli
- 国际申请: PCT/US2009/035272 WO 20090226
- 主分类号: A61K35/28
- IPC分类号: A61K35/28 ; C12N5/071 ; C12N5/10 ; C12M3/00 ; A61P9/10 ; A61P9/00
摘要:
Disclosed are methods for inducing cardiomyogenic differentiation in cells that are competent for differentiation along the cardiomyogenic lineage such as certain unfractionated bone marrow mononuclear cells (BMMNCs). In some embodiments, the methods include contacting a plurality of unfractionated, density gradient-separated BMMNCs with a cardiomyocyte differentiation-inducing amount of a Wnt11 gene product for a time and under conditions sufficient to induce cardiomyocyte differentiation in at least a subset of the BMMNCs. Also provided are methods for treating an injury to cardiac tissue in a subject using cells that have been induced to differentiate along the cardiomyogenic lineage, recombinant host cells comprising an expression vector that encodes a Wnt11 polypeptide or a functional fragment thereof, and systems for inducing cardiomyogenic differentiation in a cultured cell.