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    CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF USE THEREFOR
    1.
    发明申请
    CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF USE THEREFOR 审中-公开
    CARDIAC MYOCYTE MORPHOGENIC COMPOSITIONS AND METHODS OF THEREFOR

    公开(公告)号：US20110052551A1

    公开(公告)日：2011-03-03

    申请号：US12919648

    申请日：2009-02-26

    申请人： Michael Patrick Flaherty ,  Buddhadeb Dawn ,  Roberto Bolli

    发明人： Michael Patrick Flaherty ,  Buddhadeb Dawn ,  Roberto Bolli

    IPC分类号： A61K35/28 ,  C12N5/071 ,  C12N5/10 ,  C12M3/00 ,  A61P9/10 ,  A61P9/00

    CPC分类号： A61K35/28 ,  A61K35/12 ,  C12N5/0657 ,  C12N2501/415 ,  C12N2502/99

    摘要： Disclosed are methods for inducing cardiomyogenic differentiation in cells that are competent for differentiation along the cardiomyogenic lineage such as certain unfractionated bone marrow mononuclear cells (BMMNCs). In some embodiments, the methods include contacting a plurality of unfractionated, density gradient-separated BMMNCs with a cardiomyocyte differentiation-inducing amount of a Wnt11 gene product for a time and under conditions sufficient to induce cardiomyocyte differentiation in at least a subset of the BMMNCs. Also provided are methods for treating an injury to cardiac tissue in a subject using cells that have been induced to differentiate along the cardiomyogenic lineage, recombinant host cells comprising an expression vector that encodes a Wnt11 polypeptide or a functional fragment thereof, and systems for inducing cardiomyogenic differentiation in a cultured cell.

    摘要翻译： 公开了用于诱导沿着心肌发生谱系分化的细胞中的心肌分化的方法，例如某些未分级的骨髓单核细胞（BMMNC）。 在一些实施方案中，所述方法包括使多个未分级，密度梯度分离的BMMNC与心肌细胞分化诱导量的Wnt11基因产物在足以在至少一个BMMNC的子集中诱导心肌细胞分化的时间和条件下接触。 还提供了用于使用沿着心肌发生谱系诱导分化的细胞来治疗受试者心脏组织损伤的方法，包含编码Wnt11多肽或其功能片段的表达载体的重组宿主细胞，以及用于诱导心肌发生的系统 培养细胞中的分化。