发明申请
US20110092691A1 Method For Filtering Nucleic Acids, In Particular From Fixed Tissue
有权
用于过滤核酸的方法,特别是来自固定组织的方法
- 专利标题: Method For Filtering Nucleic Acids, In Particular From Fixed Tissue
- 专利标题(中): 用于过滤核酸的方法,特别是来自固定组织的方法
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申请号: US12997903申请日: 2009-06-18
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公开(公告)号: US20110092691A1公开(公告)日: 2011-04-21
- 发明人: Heike Euting , Guido Hennig , Kerstin Bohmann
- 申请人: Heike Euting , Guido Hennig , Kerstin Bohmann
- 申请人地址: US NY Tarrytown
- 专利权人: SIEMENS HEALTHCARE DIAGNOSTICS INC.
- 当前专利权人: SIEMENS HEALTHCARE DIAGNOSTICS INC.
- 当前专利权人地址: US NY Tarrytown
- 优先权: EP102008029356.3 20080620
- 国际申请: PCT/EP09/57561 WO 20090618
- 主分类号: C07H21/00
- IPC分类号: C07H21/00 ; H01F1/00
摘要:
The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.
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