公开(公告)号：US20110092691A1

公开(公告)日：2011-04-21

申请号：US12997903

申请日：2009-06-18

申请人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

发明人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

IPC分类号： C07H21/00 ,  H01F1/00

CPC分类号： C12N15/1013

摘要： The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

摘要翻译： 本发明涉及一种过滤核酸的方法，用于实施本发明方法的试剂盒以及用于过滤生物样品的磁性颗粒的新用途。 根据本发明的方法包括以下步骤：a）将样品保持在水溶液中; b）裂解样品; c）分离细胞碎片; 和d）从溶液中分离核酸，步骤（a）至（c）在非离液性条件下进行。

公开(公告)号：US20110245483A1

公开(公告)日：2011-10-06

申请号：US13133182

申请日：2009-11-20

申请人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

发明人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

IPC分类号： C07H1/06 ,  B65G47/22

CPC分类号： C12Q1/6806 ,  C07B63/00 ,  C12N15/1013 ,  Y10T137/8376

摘要： The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

摘要翻译： 本发明涉及核酸纯化方法，用于实施本发明方法的试剂盒和用于纯化生物样品的磁性颗粒的新应用。 根据本发明的方法包括以下步骤：a）将样品容纳在水溶液中的第一样品容器中并在非离液状态下裂解样品; 在溶液中悬浮第一磁性颗粒并将第一样品容器插入样品容器保持器中，其中样品容器插入与样品容器保持器相关联的环形磁体的环形内部空间中; 将溶液与磁性颗粒分离; 并从溶液中分离核酸。

公开(公告)号：US08846897B2

公开(公告)日：2014-09-30

申请号：US12997903

申请日：2009-06-18

申请人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

发明人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1013

摘要： The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

摘要翻译： 本发明涉及一种过滤核酸的方法，用于实施本发明方法的试剂盒以及用于过滤生物样品的磁性颗粒的新用途。 根据本发明的方法包括以下步骤：a）将样品保持在水溶液中; b）裂解样品; c）分离细胞碎片; 和d）从溶液中分离核酸，步骤（a）至（c）在非离液性条件下进行。

公开(公告)号：US08703931B2

公开(公告)日：2014-04-22

申请号：US13133182

申请日：2009-11-20

申请人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

发明人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

IPC分类号： C07H21/00

CPC分类号： C12Q1/6806 ,  C07B63/00 ,  C12N15/1013 ,  Y10T137/8376

摘要： The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

摘要翻译： 本发明涉及核酸纯化方法，用于实施本发明方法的试剂盒和用于纯化生物样品的磁性颗粒的新应用。 根据本发明的方法包括以下步骤：a）将样品容纳在水溶液中的第一样品容器中并在非离液状态下裂解样品; 在溶液中悬浮第一磁性颗粒并将第一样品容器插入样品容器保持器中，其中样品容器插入与样品容器保持器相关联的环形磁体的环形内部空间中; 将溶液与磁性颗粒分离; 并从溶液中分离核酸。