发明申请
- 专利标题: DEVICES AND PROCESSES FOR ANALYZING NUCLEIC ACID DAMAGE AND REPAIR USING ELECTROPHORESIS
- 专利标题(中): 用于分析核酸损伤和使用电泳修复的装置和方法
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申请号: US13547708申请日: 2012-07-12
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公开(公告)号: US20120277118A1公开(公告)日: 2012-11-01
- 发明人: Sangeeta N. Bhatia , Bevin P. Engelward , David K. Wood , David M. Weingeist
- 申请人: Sangeeta N. Bhatia , Bevin P. Engelward , David K. Wood , David M. Weingeist
- 申请人地址: US MA Cambridge
- 专利权人: Massachusetts Institute of Technology
- 当前专利权人: Massachusetts Institute of Technology
- 当前专利权人地址: US MA Cambridge
- 主分类号: C40B30/06
- IPC分类号: C40B30/06 ; C40B60/08
摘要:
Systems, methods, and devices are provided for assessing DNA damage and repair in cells by measuring DNA migration under electrophoresis. In one exemplary embodiment, a microarray configured to hold cells in a predetermined spatial relationship is employed to improve accuracy, speed, and reliability of such measurements. In another embodiment, a self-contained cassette having a matrix material disposed therein can be used to create a substantially uniform environment for analyzing DNA damage and repair. Fluid can be circulated through the cell to assist in creating spatial patterns on the matrix material, or alternatively, the matrix material can already include a microarray pattern disposed thereon. Various methods and systems that take advantage of such microarrays and cassettes are also provided.