公开(公告)号：US20180080020A1

公开(公告)日：2018-03-22

申请号：US15694108

申请日：2017-09-01

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/68 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  B01L7/00 ,  B01F3/08

CPC分类号： B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US20170354946A1

公开(公告)日：2017-12-14

申请号：US15669080

申请日：2017-08-04

申请人： The University Court of the University of Glasgow

发明人： Leroy CRONIN

IPC分类号： B01J19/00 ,  C40B50/04 ,  C40B60/08

CPC分类号： B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/007 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00738 ,  B01J2219/00747 ,  B01J2219/00756 ,  C40B50/04 ,  C40B60/08

摘要： The invention provides a method for preparing a compound or a product having one or more characteristics that meet or exceed a user specification, the process comprising the step of selecting a first combination of chemical inputs, optionally together with physical inputs, and supplying those inputs to a reaction space, thereby to generate a first product; analysing one or more characteristics of the product generated; comparing the one or more characteristics against a user specification; using a genetic algorithm selecting a second combination of chemical inputs, optionally together with physical inputs, wherein the second combination differs from the first combination, and supplying those inputs to the reaction space, thereby to generate a second product; analysing one or more characteristics of the second product generated; comparing the one or more characteristics generated against the user specification; repeating the selecting and analysing steps for further individual combinations of chemical and/or physical inputs, to provide an array of products wherein the flow chemistry system operates continuously to provide the first, second and further products, thereby to identify one or more products meeting or exceeding the user specification.

公开(公告)号：US09534216B2

公开(公告)日：2017-01-03

申请号：US14248991

申请日：2014-04-09

申请人： Raindance Technologies, Inc.

发明人： Darren R. Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： G01N15/10 ,  C12Q1/68 ,  G01N21/00 ,  G01N21/21 ,  C12N15/10 ,  B01F13/00 ,  B01J19/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/68 ,  G01N33/543 ,  G01N33/50 ,  G01N33/00 ,  B01L3/00 ,  B01F3/08 ,  G01N33/542 ,  G01N21/64 ,  B01L7/00

CPC分类号： C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

摘要翻译： 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 本发明提供了含有唯一标记的细胞，酶，核酸等的含水基乳液，其中乳液还包含引物，标记，探针和其它反应物。 油基载体流体在微流体装置上包封乳剂库，使得连续通道提供不混溶流体的流动，以实现乳液库的汇集，聚结，混合，分选，检测等。

公开(公告)号：US08420017B2

公开(公告)日：2013-04-16

申请号：US12872498

申请日：2010-08-31

申请人： Robert C Jones ,  Jing Wang ,  Andrew May ,  David Cohen

发明人： Robert C Jones ,  Jing Wang ,  Andrew May ,  David Cohen

IPC分类号： B01J19/00 ,  B01L3/00 ,  B01L99/00 ,  F04B19/00 ,  B01J8/00 ,  C40B60/08

CPC分类号： B01J19/00 ,  B01F5/0085 ,  B01F5/0602 ,  B01F13/0069 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00355 ,  B01J2219/00389 ,  B01J2219/00418 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0605 ,  B01L2400/0655

摘要： A microfluidic device is described that has a slug mixing arrangement. A reaction well has an input flow channel with a first valve near the reaction well, and a second valve further from the reaction well. A fluid source is connected to the segment in between the two valves. A second fluid source is connected behind the second valve. The channel between the two valves receives the first fluid by blind filling when the two valves are closed. The reaction well receives the first fluid followed by the second fluid when the first and second valves are open.

摘要翻译： 描述了具有块状混合装置的微流体装置。 反应井具有在反应井附近具有第一阀的输入流动通道，以及进一步离开反应井的第二阀。 流体源连接到两个阀之间的段。 第二流体源连接在第二阀的后面。 当两个阀关闭时，两个阀门之间的通道通过盲填充接收第一流体。 当第一和第二阀打开时，反应井接收第一流体，随后是第二流体。

公开(公告)号：US20130035257A1

公开(公告)日：2013-02-07

申请号：US13567801

申请日：2012-09-10

申请人： Linfen YU ,  Karen C. CHEUNG

发明人： Linfen YU ,  Karen C. CHEUNG

IPC分类号： C40B30/08 ,  B32B37/14 ,  C40B40/02 ,  C12N11/02 ,  C40B50/06 ,  C40B60/08

CPC分类号： C12N11/04 ,  B01J2219/00527 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2400/086 ,  C40B60/08 ,  G01N33/5032

摘要： Methods of isolating at least one cell of interest, methods of making fixed arrays, arrays comprising a glass substrate bonded to a patterned siloxane structure having inlets, outlets and microchannels, array kits, and methods of making microfluidic apparati are provided in the present application.

摘要翻译： 在本申请中提供了分离至少一个感兴趣的细胞的方法，制备固定阵列的方法，包含结合到具有入口，出口和微通道的图案化硅氧烷结构的玻璃基底的阵列，阵列试剂盒和制备微流体装置的方法。

公开(公告)号：US08357490B2

公开(公告)日：2013-01-22

申请号：US12841708

申请日：2010-07-22

申请人： Thomas Froehlich ,  Martin Gutekunst ,  Dieter Heindl ,  Angelika Roesler ,  Rudolf Seibl

发明人： Thomas Froehlich ,  Martin Gutekunst ,  Dieter Heindl ,  Angelika Roesler ,  Rudolf Seibl

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C40B60/02 ,  C40B60/04 ,  C40B60/08

CPC分类号： G01N35/028 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00315 ,  B01J2219/00364 ,  B01J2219/00423 ,  B01J2219/00454 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L7/52 ,  C40B60/14 ,  G01N35/1002 ,  G01N2035/00326 ,  G01N2035/00356

摘要： An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.

摘要翻译： 公开了一种用于寡核苷酸合成和PCR的综合仪器及其系统及方法。 综合仪器基本上由两个独立的模块组成。 第一模块是寡核苷酸引物和/或寡核苷酸杂交探针等寡核苷酸的化学从头合成的单元。 第二个模块是用于实时进行分析聚合酶链反应扩增的单元，即qPCR。 两个模块可操作地彼此连接，使得用户可以将待分析的核酸样品加载到整合的仪器中，并且通过在没有先前的外部合成寡核苷酸扩增引物的情况下编程仪器进行PCR反应。

公开(公告)号：US20120202711A1

公开(公告)日：2012-08-09

申请号：US13192268

申请日：2011-07-27

申请人： Tae Sun Kim ,  Keum Soo Song ,  Jung Hoon Kim ,  Hyung Sub Kim

发明人： Tae Sun Kim ,  Keum Soo Song ,  Jung Hoon Kim ,  Hyung Sub Kim

IPC分类号： C40B40/06 ,  C40B50/14 ,  C40B60/08

CPC分类号： C07C251/24 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  C07C2603/92

摘要： The present invention relates to novel iminecalixarene derivatives, method of preparation thereof, and self-assembled monolayer prepared by the method, fixing method of oligo-DNA by using the self-assembled monolayer, and oligo-DNA chip prepared by the method. Also, the present invention relates to novel aminocalixarene derivatives, method of preparation thereof, and self-assembled monolayer prepared by the method, fixing method of oligo-DNA wherein the oligo-DNA is voluntarily fixed by molecular recognition on said self-assembled monolayer in a liquid phase, and oligo-DNA chip prepared by the method.

摘要翻译： 本发明涉及新颖的亚氨基癸烷衍生物，其制备方法和通过该方法制备的自组装单层，通过使用自组装单层固定寡DNA的方法，以及通过该方法制备的寡核苷酸芯片。 此外，本发明涉及新型氨基焦虑衍生物，其制备方法和通过该方法制备的自组装单层，其中通过分子识别在所述自组装单层上自愿固定寡DNA的寡DNA的固定方法 液相和通过该方法制备的寡核苷酸芯片。

公开(公告)号：US20110312617A1

公开(公告)日：2011-12-22

申请号：US13150156

申请日：2011-06-01

申请人： Mehdi Azimi ,  Kia Silverbrook

发明人： Mehdi Azimi ,  Kia Silverbrook

IPC分类号： C40B60/08

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A microsystems technology (MST) device for contactless spotting of oligonucleotide probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the MST device having a monolithic substrate having a reservoir side and an ejector side opposite the reservoir side, an array of reservoirs formed in the reservoir side, and, an array of ejectors formed on the ejector side, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, wherein, the ejectors are configured to eject droplets containing the oligonucleotide probes from the corresponding reservoir onto the surface.

摘要翻译： 一种用于将寡核苷酸探针非接触点样的微系统技术（MST）装置，所述探针具有与待生物样品中鉴定的靶核酸序列互补的核酸序列，所述MST装置具有具有储存器侧的整体式基底 以及与储存器侧相对的喷射器侧，在储存器侧形成的储存器阵列，以及形成在喷射器侧的喷射器阵列，每个喷射器分别构造成与相应的一个储存器流体连通，其中 喷射器构造成将含有寡核苷酸探针的液滴从相应的储存器喷射到表面上。

公开(公告)号：US20110251109A1

公开(公告)日：2011-10-13

申请号：US12572230

申请日：2009-10-01

申请人： Xiaochuan Zhou ,  Tiecheng Zhou ,  David Sun

发明人： Xiaochuan Zhou ,  Tiecheng Zhou ,  David Sun

IPC分类号： C40B60/08

CPC分类号： B01J19/0046 ,  B01J19/0093 ,  B01J2219/00279 ,  B01J2219/00306 ,  B01J2219/00313 ,  B01J2219/00322 ,  B01J2219/00351 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00734 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00936 ,  B01J2219/00943 ,  B01L3/5025 ,  B82Y30/00 ,  C07K1/045 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  Y10T29/49

摘要： Fluidic methods and devices for conducting parallel chemical reactions are disclosed. The methods are based on the use of in situ photogenerated reagents such as photogenerated acids, photogenerated bases, or any other suitable chemical compounds that produce active reagents upon light radiation. The present invention describes devices and methods for performing a large number of parallel chemical reactions without the use of a large number of valves, pumps, and other complicated fluidic components. The present invention provides microfluidic devices that contain a plurality of microscopic vessels for carrying out discrete chemical reactions. Other applications may include the preparation of microarrays of DNA and RNA oligonucleotides, peptides, oligosaccharides, phospholipids and other biopolymers on a substrate surface for assessing gene sequence information, screening for biological and chemical activities, identifying intermolecular complex formations, and determining structural features of molecular complexes.

摘要翻译： 公开了用于进行平行化学反应的流体方法和装置。 这些方法基于使用原位光生试剂，例如光生酸，光生碱或在光辐射时产生活性试剂的任何其它合适的化合物。 本发明描述了在不使用大量阀，泵和其它复杂流体组分的情况下进行大量并行化学反应的装置和方法。 本发明提供了包含用于进行离散化学反应的多个微观容器的微流体装置。 其他应用可能包括在底物表面上制备DNA和RNA寡核苷酸，肽，寡糖，磷脂和其他生物聚合物的微阵列，用于评估基因序列信息，筛选生物和化学活性，鉴定分子间复合物形成并确定分子的结构特征 复合物。

公开(公告)号：US20110152108A1

公开(公告)日：2011-06-23

申请号：US12921144

申请日：2009-03-06

申请人： Colin J.H. Brenan ,  Thomas B. Morrison ,  Tanya S. Kanigan

发明人： Colin J.H. Brenan ,  Thomas B. Morrison ,  Tanya S. Kanigan

IPC分类号： C40B30/00 ,  C40B60/08 ,  C40B50/06

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50851 ,  B01L3/50857 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/12 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2300/1844 ,  B01L2300/185 ,  B01L2300/1894

摘要： One aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a conductive member for heating the interior volume. Another aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a plurality of conductive members for heating an interior volume. Another aspect of the invention provides a container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a first conductive member located in the interior volume and adapted to contact a first end of the microfluidic array.

摘要翻译： 本发明的一个方面提供用于热循环多个微流体阵列中的样品的容器。 容器包括限定内部容积的多个壁和用于加热内部空间的导电构件。 本发明的另一方面提供了用于在微流体阵列中热循环多个样品的容器。 容器包括限定内部容积的多个壁和用于加热内部容积的多个导电构件。 本发明的另一方面提供了一种用于在微流体阵列中热循环多个样品的容器。 容器包括限定内部容积的多个壁和位于内部容积中并适于接触微流体阵列的第一端的第一导电构件。