发明申请
US20130131321A1 LIGANDS FOR ANTIBODY AND Fc-FUSION PROTEIN PURIFICATION BY AFFINITY CHROMATOGRAPHY
审中-公开
通过亲和色谱法进行抗体和Fc融合蛋白纯化的配体
- 专利标题: LIGANDS FOR ANTIBODY AND Fc-FUSION PROTEIN PURIFICATION BY AFFINITY CHROMATOGRAPHY
- 专利标题(中): 通过亲和色谱法进行抗体和Fc融合蛋白纯化的配体
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申请号: US13813860申请日: 2011-08-03
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公开(公告)号: US20130131321A1公开(公告)日: 2013-05-23
- 发明人: Holger Bittermann , Klaus Burkert , Marc Arnold , Oliver Keil , Thomas Neumann , Inge Ott , Kristina Schmidt , Daniel Schwizer , Renate Sekul
- 申请人: Holger Bittermann , Klaus Burkert , Marc Arnold , Oliver Keil , Thomas Neumann , Inge Ott , Kristina Schmidt , Daniel Schwizer , Renate Sekul
- 申请人地址: DE Heidelberg
- 专利权人: GRAFFINITY PHARMACEUTICALS GMBH
- 当前专利权人: GRAFFINITY PHARMACEUTICALS GMBH
- 当前专利权人地址: DE Heidelberg
- 优先权: EP10008089.4 20100803
- 国际申请: PCT/EP11/63392 WO 20110803
- 主分类号: C07K1/22
- IPC分类号: C07K1/22 ; C07D405/04 ; C07D409/04 ; C07D231/14 ; C07D231/56 ; C07D213/82 ; C07D403/04 ; C07D209/42 ; C07D261/20 ; C07D307/87 ; C07D217/26 ; C07D513/04 ; C07D471/04 ; C07D417/04 ; C07D413/04 ; C07D235/24 ; C07D487/04 ; C07D261/18 ; C07D271/06 ; C07D277/68 ; C07D277/56 ; C08B37/00
摘要:
The present invention relates to the use for affinity purification of an antibody or an fragment of an antibody, of a ligand-substituted matrix comprising a support material and at least one ligand covalently bonded to the support material, the ligand being represented by formula (I) wherein Sp is a spacer group; v is 0 or 1; Am is an amide group —NR1—C(O)—, and wherein either NR1 is attached to Ar1 and —C(O)— is attached to Ar2, or —C(O)— is attached to Ar1 and NR1 is attached to Ar2; and R1 is hydrogen or C1 to C4 alkyl, preferably hydrogen or methyl; and more preferably hydrogen; Ar1 is a divalent 5- or 6-membered substituted or unsubstituted aromatic ring; Ar2 is 5- or 6-membered heterocyclic aromatic ring which is (a) attached to a further 5- or 6-membered aromatic ring via a single bond; or (b) fused to a further 5- or 6-membered aromatic ring as part of a multicyclic ring system; or (c) attached to at least one substituent selected from C1 to C4 alkyl; C2 to C4 alkenyl; C2 to C4 alkynyl; a halogen; C1 to C4 haloalkyl; hydroxyl-substituted C1 to C4 alkyl; C1 to C4 alkoxy; hydroxyl-substituted C1 to C4 alkoxy; C1 to C4 alkylamino; C1 to C4 alkylthio; and combinations thereof.