The present invention relates to a method for producing male sterile plant, a mutated nucleotide molecule comprising a nucleotide sequence of the transcription factor bHLH142 and an inserted T-DNA segment, and a novel transformed plant cell and a male-sterile mutant plant comprising the mutated nucleotide molecule, in which the transcription factor bHLH142 is not expressed. The present invention also relates to a novel reversible male sterile transgenic plant, wherein the transcription factor bHLH142 is overexpressed, and its preparation method. The bHLH gene is tissue specifically expresses in the anther and it plays a pivotal role in pollen development. Both the male sterile and reversible male sterile transgenic plants showed a completely male sterile phenotype, but the fertility of the reversible male sterile transgenic plant can be restored under low temperature.