Invention Application
US20160032338A1 METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION
有权
从ASPERGILLUS FUMIGATUS和ISOAMYLASE使用ALPHA-AMYLASE进行鉴定的方法
- Patent Title: METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION
- Patent Title (中): 从ASPERGILLUS FUMIGATUS和ISOAMYLASE使用ALPHA-AMYLASE进行鉴定的方法
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Application No.: US14649173Application Date: 2013-12-05
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Publication No.: US20160032338A1Publication Date: 2016-02-04
- Inventor: Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhengong Zhang
- Applicant: DANISCO US INC.
- Priority: CNPCT/CN2012/086654 20121214
- International Application: PCT/US2013/073317 WO 20131205
- Main IPC: C12P19/14
- IPC: C12P19/14 ; C12P19/02 ; C12C11/00 ; C12N9/44

Abstract:
A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Public/Granted literature
- US09765374B2 Method of using α-amylase from Aspergillus fumigatus and isoamylase for saccharification Public/Granted day:2017-09-19
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