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公开(公告)号：US09765374B2
公开(公告)日：2017-09-19
申请号：US14649173
申请日：2013-12-05
Applicant: Danisco US Inc.
Inventor： Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhengong Zhang
IPC: C12P7/06 , C12P19/14 , A21D8/04 , C11D3/386 , D06L1/06 , C12C5/00 , C12N9/30 , C12N9/44 , C12C11/00 , C12P19/02 , A23L7/104
CPC classification number: C12P19/14 , A21D8/042 , A23L7/107 , A23V2002/00 , C11D3/386 , C12C5/004 , C12C11/003 , C12N9/242 , C12N9/246 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01068 , D06L1/06 , Y02E50/17
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
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2.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION 有权使用来自青蒿素的ALPHA-AMYLASE进行分离的方法公开(公告)号：US20130323798A1
公开(公告)日：2013-12-05
申请号：US13888303
申请日：2013-05-06
Applicant: Danisco US Inc.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 从曲霉菌（AcAmyl）提供真菌α-淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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公开(公告)号：US20210047664A1
公开(公告)日：2021-02-18
申请号：US17002091
申请日：2020-08-25
Applicant: DANISCO US INC
Inventor： JING GE , Ling Hua , Sung Ho Lee , Jalsen Li , Jayarama K. Shetty , Zhongmei Tang , Bo Zhang , Kun Zhong
Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
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4.发明申请公开(公告)号：US20160186102A1
公开(公告)日：2016-06-30
申请号：US14907707
申请日：2014-09-19
Applicant: DANISCO US INC.
Inventor： Guoqing Liu , Ling Hua , Zhen Qian , Danfeng Song , Zhongmei Tang , Xi Zhiyong , Bo Zhang , Zhengzheng Zou , Wei Xu , Richard R. Bott
CPC classification number: C11D3/386 , C12N9/2417 , C12Y302/01001
Abstract: Disclosed are compositions and methods relating to alpha-amylase from a subset of Exiguobacterium. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Abstract translation: 公开了与来自Exiguobacterium的子集的α-淀粉酶有关的组合物和方法。 组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它应用中的淀粉污渍，用于织物加工（例如脱浆），用于改善消化率的动物饲料中，以及烘烤和酿造 。
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5.发明授权公开(公告)号：US09365871B2
公开(公告)日：2016-06-14
申请号：US14561085
申请日：2014-12-04
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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Patent Agency Ranking
6.发明申请TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF 审中-公开表达来自青蒿素的谷氨酰胺酶的TRICHODERMA REESEI HOST细胞及其使用方法公开(公告)号：US20160115509A1
公开(公告)日：2016-04-28
申请号：US14649167
申请日：2013-11-21
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Sung Ho Lee , Jalsen Li , Jayarama K. Shetty , Zhongmei Tang , Bo Zhang , Kun Zhong
CPC classification number: C12P19/14 , C12N9/2428 , C12P19/02 , C12Y302/01003 , Y02E50/17
Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
Abstract translation: 提供了在里氏木霉宿主细胞（AfGATR）中表达的来自烟曲霉的真菌葡糖淀粉酶。 里氏木霉宿主细胞以更高或至少相当的水平将AfGATR表达为天然表达的AfGA烟曲霉（Aspergillus fumigatus）。 包括AfGA1TR和AfGA2TR的AfGATR在升高的温度和低pH下表现出高活性，因此在α-淀粉酶例如曲霉曲霉α-淀粉酶（AkAA）的存在下，可以有效地使用AfGATR。 与由烟曲霉中表达的黑曲霉葡糖淀粉酶（AnGA）或天然AfGA催化的糖化产物相比，AfGATR有利地将淀粉糖化催化剂显着富集DP1（即葡萄糖）的寡糖组合物。 AfGATR如AfGA1TR，AfGA2TR或其变体可以以比AnGA和天然表达的AfGA更低的剂量使用以产生相当水平的葡萄糖。
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7.发明申请METHOD OF USING ALPHA-AMYLASE FROM TALAROMYCES EMERSONII FOR SACCHARIFICATION 审中-公开使用来自TALAROMESCES EMERSONII的ALPHA-AMYLASE进行鉴定的方法公开(公告)号：US20150240223A1
公开(公告)日：2015-08-27
申请号：US14433941
申请日：2013-09-17
Applicant: DANISCO US INC.
Inventor： Ling Hua , Zhongmei Tang , Casper Willem Vroemen , Bo Zhang , Zhenghong Zhang
CPC classification number: C12N9/242 , A21D8/042 , A23L29/35 , C11D3/386 , C12C5/004 , C12C7/04 , C12C7/14 , C12C11/003 , C12C11/07 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06L1/12 , D06L1/14 , D06M16/003 , Y02E50/16 , Y02E50/17
Abstract: A fungal alpha amylase is provided from Talaromyces emersonii (TeAmy1), along with variants of the same. TeAmy1 has an optimal pH of 3.5 and is operable over a temperature range of at least 30 75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. TeAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α淀粉酶从艾美（Talaromyces emersonii）（TeAmy1）提供，以及相同的变体。 TeAmy1具有3.5的最佳pH，并且在至少3075℃的温度范围内是可操作的，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇方法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，TeAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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8.发明申请公开(公告)号：US20190177757A1
公开(公告)日：2019-06-13
申请号：US16281989
申请日：2019-02-21
Applicant: DANISCO US INC
Inventor： Jing Ge , Ling Hua , Sung Ho Lee , Jalsen Li , Jayarama K Shetty , Zhongmei Tang , Bo Zhang , Kun Zhong
CPC classification number: C12P19/14 , C12N9/2428 , C12P19/02 , C12Y302/01003 , Y02E50/17
Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
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9.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND PULLULANASE FOR SACCHARIFICATION 审中-公开使用来自青蒿素和富勒烯的ALPHA-AMYLASE进行分离的方法公开(公告)号：US20160010128A1
公开(公告)日：2016-01-14
申请号：US14649807
申请日：2013-12-06
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang
CPC classification number: C12P19/14 , A21D8/042 , A23L2/382 , A23L7/107 , A23L29/35 , C11D3/386 , C12C5/004 , C12C7/04 , C12C7/16 , C12C11/003 , C12C12/04 , C12N9/242 , C12P7/06 , C12P7/14 , C12P19/02 , C12P2203/00 , C13K1/06 , C13K11/00 , D06L1/06 , Y02E50/17
Abstract: A fungal alpha-amylase is provided from Aspergillus terreus (AtAmyl). AtAmyl has an optimal pH of 4.5 and is operable at 30 75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 由Aspergillus terreus（AtAmyl）提供真菌α-淀粉酶。 AtAmyl具有4.5的最佳pH，并且在30±75℃下可操作，使酶在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α-淀粉酶催化的糖化产物相比，AtAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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10.发明授权Method of using alpha-amylase from Aspergillus clavatus for saccharification 有权使用来自曲霉菌的α-淀粉酶进行糖化的方法公开(公告)号：US08945889B2
公开(公告)日：2015-02-03
申请号：US13888303
申请日：2013-05-06
Applicant: Danisco US Inc.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmyl）提供。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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