Invention Application
US20160115225A1 Methods of Reducing Methylglyoxal (MGO) Modification of Recombinant Proteins in Cell Culture
审中-公开
减少甲基乙二醛（MGO）细胞培养重组蛋白修饰的方法
- Patent Title: Methods of Reducing Methylglyoxal (MGO) Modification of Recombinant Proteins in Cell Culture
- Patent Title (中): 减少甲基乙二醛（MGO）细胞培养重组蛋白修饰的方法
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Application No.: US14922088Application Date: 2015-10-23
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Publication No.: US20160115225A1Publication Date: 2016-04-28
- Inventor: Christopher M. Chumsae , Kartik Subramanian , Yun ("Frank") Zhang
- Applicant: AbbVie Inc.
- Assignee: AbbVie Inc.
- Current Assignee: AbbVie Inc.
- Main IPC: C07K16/24
- IPC: C07K16/24
Abstract:
Methods are provided for controlling the levels of acidic species when a protein of interest is expressed by host cells. One particular example of an acidic species is methylglyoxal (MGO). Reduction of acidic species such as MGO may be achieved by regulating the glucose consumption of the cell culture or by modulating the cysteine/glutathione metabolic pathways.
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