Invention Application
- Patent Title: CAS9 NUCLEASE PLATFORM FOR MICROALGAE GENOME ENGINEERING
- Patent Title (中): 用于微藻基因工程的CAS9核平台
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Application No.: US15103773Application Date: 2014-12-12
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Publication No.: US20160304893A1Publication Date: 2016-10-20
- Inventor: Fayza DABOUSSI , Marine BEURDELEY , Alexandre JUILLERAT
- Applicant: CELLECTIS
- Priority: DKPA201370772 20131213
- International Application: PCT/EP2014/077508 WO 20141212
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C12N9/22
Abstract:
The present invention relates to a method of genome engineering in microalgae using the Cas9/CRISPR system. In particular, the present invention relates to methods of delivering RNA guides via cell penetrating peptides in microalgae, preferably in stable integrated Cas9 microalgae. The present invention also relates to kits and isolated cells comprising Cas9, split Cas9 or guide RNA and Cas9-fused cell-penetrating peptides. The present invention also relates to isolated cells obtained by the methods of the invention.
Information query
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