公开(公告)号：US20160304893A1

公开(公告)日：2016-10-20

申请号：US15103773

申请日：2014-12-12

Applicant: CELLECTIS

Inventor： Fayza DABOUSSI ,  Marine BEURDELEY ,  Alexandre JUILLERAT

IPC: C12N15/82 ,  C12N9/22

CPC classification number: C12N15/8213 ,  C12N9/22

Abstract: The present invention relates to a method of genome engineering in microalgae using the Cas9/CRISPR system. In particular, the present invention relates to methods of delivering RNA guides via cell penetrating peptides in microalgae, preferably in stable integrated Cas9 microalgae. The present invention also relates to kits and isolated cells comprising Cas9, split Cas9 or guide RNA and Cas9-fused cell-penetrating peptides. The present invention also relates to isolated cells obtained by the methods of the invention.

Abstract translation: 本发明涉及使用Cas9 / CRISPR系统的微藻中的基因组工程方法。 特别地，本发明涉及通过微藻中的细胞穿透肽递送RNA引导物的方法，优选在稳定的综合Cas9微藻中。 本发明还涉及包含Cas9，裂解Cas9或引导RNA和Cas9融合的细胞穿透肽的试剂盒和分离的细胞。 本发明还涉及通过本发明的方法获得的分离的细胞。

公开(公告)号：US20160355875A1

公开(公告)日：2016-12-08

申请号：US15028644

申请日：2014-10-10

Applicant: CELLECTIS

Inventor： Julien VALTON ,  Alexandre JUILLERAT ,  Fayza DABOUSSI ,  Marine BEURDELEY ,  Claudia BERTONATI ,  Philippe DUCHATEAU

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6841 ,  G01N33/5308 ,  C12Q2522/101 ,  C12Q2565/531

Abstract: The present invention relates to a method for the detection of a specific nucleic acid. Specifically, the invention provides a method and kits for detecting the presence of a specific nucleic acid using engineered DNA-binding domains such as Transcription Activator Like-Effector (TALE) domain or modular base-per-base binding domains (MBBBD). The method of the invention is particularly useful for in vitro diagnostic application.

Abstract translation: 本发明涉及用于检测特定核酸的方法。 具体地，本发明提供了使用工程化的DNA结合结构域如转录激活子效应（TALE）结构域或模块化碱基结合结构域（MBBBD）来检测特异性核酸的存在的方法和试剂盒。 本发明的方法对于体外诊断应用特别有用。