公开(公告)号：US20160130599A1

公开(公告)日：2016-05-12

申请号：US14899107

申请日：2014-06-25

Applicant: CELLECTIS

Inventor： Philippe DUCHATEAU ,  Fayza DABOUSSI ,  David SOURDIVE ,  Jean-Charles EPINAT

IPC: C12N15/82 ,  C12P7/64

CPC classification number: C12N15/8247 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1241 ,  C12N9/16 ,  C12N15/79 ,  C12P7/64 ,  C12P7/6427 ,  C12Y101/01008 ,  C12Y103/01009 ,  C12Y114/19006 ,  C12Y203/01 ,  C12Y207/07009 ,  C12Y301/02022

Abstract: The invention provides engineered diatoms and methods of producing oil using diatoms. The invention also provides methods of modifying the lipids quantity and/or quality produced by diatom organisms through genome engineering. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured from such modified diatoms.

Abstract translation: 本发明提供工程硅藻和使用硅藻生产油的方法。 本发明还提供了通过基因组工程改变由硅藻生物产生的脂质量和/或质量的方法。 还提供油​​，燃料，油化学品，化学前体和由这种改性硅藻制造的其它化合物。

公开(公告)号：US20160355875A1

公开(公告)日：2016-12-08

申请号：US15028644

申请日：2014-10-10

Applicant: CELLECTIS

Inventor： Julien VALTON ,  Alexandre JUILLERAT ,  Fayza DABOUSSI ,  Marine BEURDELEY ,  Claudia BERTONATI ,  Philippe DUCHATEAU

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6841 ,  G01N33/5308 ,  C12Q2522/101 ,  C12Q2565/531

Abstract: The present invention relates to a method for the detection of a specific nucleic acid. Specifically, the invention provides a method and kits for detecting the presence of a specific nucleic acid using engineered DNA-binding domains such as Transcription Activator Like-Effector (TALE) domain or modular base-per-base binding domains (MBBBD). The method of the invention is particularly useful for in vitro diagnostic application.

Abstract translation: 本发明涉及用于检测特定核酸的方法。 具体地，本发明提供了使用工程化的DNA结合结构域如转录激活子效应（TALE）结构域或模块化碱基结合结构域（MBBBD）来检测特异性核酸的存在的方法和试剂盒。 本发明的方法对于体外诊断应用特别有用。

公开(公告)号：US20160312233A1

公开(公告)日：2016-10-27

申请号：US15103751

申请日：2014-12-12

Applicant: CELLECTIS

Inventor： Philippe DUCHATEAU ,  Fayza DABOUSSI

IPC: C12N15/79 ,  C12N15/90 ,  C12N9/22 ,  C12N15/10

CPC classification number: C12N15/79 ,  C12N9/22 ,  C12N15/102 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8213 ,  C12N15/902 ,  C12N2800/80 ,  C12Y301/00

Abstract: The invention relates to a method to select transformed cells. In particular, the present invention relates to the use of a nuclease engineered to inactivate selectable marker which confers cell resistance to a toxic compound. The present invention relates to methods of modifying genome of a cell, preferably an algal cell comprising the present selection step. The present invention also relates to specific engineered nucleases, polynucleotides, vectors encoding thereof, kits and isolated cells comprising said nuclease.

Abstract translation: 本发明涉及一种选择转化细胞的方法。 特别地，本发明涉及工程化的核酸酶在使有毒化合物赋予细胞耐药性的选择性标记失活的用途。 本发明涉及修饰细胞基因组的方法，所述方法优选包含本选择步骤的藻细胞。 本发明还涉及特异性工程化核酸酶，多核苷酸，编码它们的载体，试剂盒和包含所述核酸酶的分离的细胞。

公开(公告)号：US20160304893A1

公开(公告)日：2016-10-20

申请号：US15103773

申请日：2014-12-12

Applicant: CELLECTIS

Inventor： Fayza DABOUSSI ,  Marine BEURDELEY ,  Alexandre JUILLERAT

IPC: C12N15/82 ,  C12N9/22

CPC classification number: C12N15/8213 ,  C12N9/22

Abstract: The present invention relates to a method of genome engineering in microalgae using the Cas9/CRISPR system. In particular, the present invention relates to methods of delivering RNA guides via cell penetrating peptides in microalgae, preferably in stable integrated Cas9 microalgae. The present invention also relates to kits and isolated cells comprising Cas9, split Cas9 or guide RNA and Cas9-fused cell-penetrating peptides. The present invention also relates to isolated cells obtained by the methods of the invention.

Abstract translation: 本发明涉及使用Cas9 / CRISPR系统的微藻中的基因组工程方法。 特别地，本发明涉及通过微藻中的细胞穿透肽递送RNA引导物的方法，优选在稳定的综合Cas9微藻中。 本发明还涉及包含Cas9，裂解Cas9或引导RNA和Cas9融合的细胞穿透肽的试剂盒和分离的细胞。 本发明还涉及通过本发明的方法获得的分离的细胞。