Invention Application
- Patent Title: POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME
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Application No.: US15254193Application Date: 2016-09-01
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Publication No.: US20160369328A1Publication Date: 2016-12-22
- Inventor: David LIGHT , Barnett B. ROSENBLUM
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C08F222/38

Abstract:
The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.
Public/Granted literature
- US09890423B2 Polymer particles, nucleic acid polymer particles and methods of making and using the same Public/Granted day:2018-02-13
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