公开(公告)号：US20150175734A1

公开(公告)日：2015-06-25

申请号：US14640009

申请日：2015-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Barnett B. ROSENBLUM

IPC: C08F251/00

CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

Abstract translation: 本公开涉及制备聚合物颗粒的方法，所述方法包括以下步骤：制备水性凝胶反应混合物; 在连续相中形成具有分散的凝胶反应混合物的水相胶束的乳液; 向连续相中加入包含至少一种聚合引发剂的引发剂油; 并在胶束中进行聚合反应。 此外，引发剂油相对于水性凝胶反应混合物的体积的体积％为约1体积％至约20体积％。 本公开还涉及制备具有相同方法步骤的核酸聚合物颗粒的方法，并且其中所述水性凝胶反应混合物包括核酸片段，例如引物。

公开(公告)号：US20160369328A1

公开(公告)日：2016-12-22

申请号：US15254193

申请日：2016-09-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Barnett B. ROSENBLUM

IPC: C12Q1/68 ,  C08F222/38

CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.