Disclosed are a method and apparatus for transmitting an uplink channel in a wireless communication system. A method for transmitting an uplink channel in a wireless communication system supporting an sTTI is performed by a terminal incapable of the simultaneous transmission of a first uplink channel and a second uplink channel, and includes when a first uplink channel region at a first sTTI overlaps a specific symbol included in a second uplink channel region at a second sTTI, transmitting the first uplink channel to a base station using at least one of a plurality of symbols included in the first uplink channel region symbol other than the specific symbol at the first sTTI and transmitting the second uplink channel to the base station using at least one symbol included in the second uplink channel region at the second sTTI. The specific symbol includes a symbol to which a DMRS related to the second uplink channel is mapped.