Invention Application
- Patent Title: METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION
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Application No.: US15417736Application Date: 2017-01-27
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Publication No.: US20170321273A1Publication Date: 2017-11-09
- Inventor: Steven T. BRENTANO , Dmitry LYAKHOV , James D. CARLSON , Norman C. NELSON , Lyle J. ARNOLD , Michael M. BECKER
- Applicant: GEN-PROBE INCORPORATED
- Main IPC: C12Q1/68
- IPC: C12Q1/68

Abstract:
Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
Public/Granted literature
- US10415092B2 Methods and compositions for nucleic acid amplification Public/Granted day:2019-09-17
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