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    INACTIVATABLE TARGET CAPTURE OLIGOMERS FOR USE IN THE SELECTIVE HYBRIDIZATION AND CAPTURE OF TARGET NUCLEIC ACID SEQUENCES
    1.
    发明申请
    INACTIVATABLE TARGET CAPTURE OLIGOMERS FOR USE IN THE SELECTIVE HYBRIDIZATION AND CAPTURE OF TARGET NUCLEIC ACID SEQUENCES 审中-公开

    公开(公告)号：US20180010172A1

    公开(公告)日：2018-01-11

    申请号：US15708039

    申请日：2017-09-18

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAN

    IPC: C12Q1/68

    Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.

    TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS
    2.
    发明申请
    TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS 审中-公开

    公开(公告)号：US20190024158A9

    公开(公告)日：2019-01-24

    申请号：US15069645

    申请日：2016-03-14

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAM

    IPC: C12Q1/68

    Abstract: The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.

    COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACID FROM MOLLICUTES
    3.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACID FROM MOLLICUTES 有权
    用于检测来自麦芽糖的核酸的组合物和方法

    公开(公告)号：US20160060685A1

    公开(公告)日：2016-03-03

    申请号：US14938630

    申请日：2015-11-11

    Applicant: Gen-Probe Incorporated

    Inventor： Shannon K. KAPLAN ,  Kristin W. LIVEZEY ,  Michael M. BECKER ,  James J. HOGAN

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

    Abstract translation: 用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。 23S rRNA或其基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。 一些寡聚体包括标签区，靶闭合区，启动子序列和/或结合部分。 样品可以来自怀疑含有Mollicutes类物种的任何来源。 优选的样品来源包括生物反应器，细胞系，细胞培养物和制药产品。

    INACTIVATABLE TARGET CAPTURE OLIGOMERS FOR USE IN THE SELECTIVE HYBRIDIZATION AND CAPTURE OF TARGET NUCLEIC ACID SEQUENCES
    4.
    发明申请
    INACTIVATABLE TARGET CAPTURE OLIGOMERS FOR USE IN THE SELECTIVE HYBRIDIZATION AND CAPTURE OF TARGET NUCLEIC ACID SEQUENCES 有权

    公开(公告)号：US20210024982A1

    公开(公告)日：2021-01-28

    申请号：US17061552

    申请日：2020-10-01

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAM

    IPC: C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/686

    Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.

    COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACID FROM MOLLICUTES
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACID FROM MOLLICUTES 审中-公开

    公开(公告)号：US20180073059A1

    公开(公告)日：2018-03-15

    申请号：US15699502

    申请日：2017-09-08

    Applicant: Gen-Probe Incorporated

    Inventor： Shannon K. KAPLAN ,  Kristin W. LIVEZEY ,  Michael M. BECKER ,  James J. HOGAN

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

    TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS
    8.
    发明申请
    TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS 审中-公开

    公开(公告)号：US20160186249A1

    公开(公告)日：2016-06-30

    申请号：US15069645

    申请日：2016-03-14

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAM

    IPC: C12Q1/68

    CPC classification number: C12Q1/6848 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2525/155

    Abstract: The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION
    9.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
    用于核酸扩增的方法和组合物

    公开(公告)号：US20130309673A1

    公开(公告)日：2013-11-21

    申请号：US13956158

    申请日：2013-07-31

    Applicant: Gen-Probe Incorporated

    Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, JR.

    IPC: C12Q1/68

    CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

    Abstract translation: 用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。 一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。 靶核酸的靶序列被预扩增以产生第一扩增产物。 第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

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