Invention Grant
- Patent Title: Methods and compositions for nucleic acid amplification
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Application No.: US15417736Application Date: 2017-01-27
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Publication No.: US10415092B2Publication Date: 2019-09-17
- Inventor: Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold , Michael M. Becker
- Applicant: GEN-PROBE INCORPORATED
- Applicant Address: US CA San Diego
- Assignee: GEN-PROBE INCORPORATED
- Current Assignee: GEN-PROBE INCORPORATED
- Current Assignee Address: US CA San Diego
- Agent Nicholas V. Sherbina; Jeffrey E. Landes
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6881 ; C12Q1/6844 ; C12Q1/6834 ; C12Q1/6865

Abstract:
Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
Public/Granted literature
- US20170321273A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION Public/Granted day:2017-11-09
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