Invention Application
- Patent Title: EXTRACELLULAR DITERPENE PRODUCTION
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Application No.: US15702308Application Date: 2017-09-12
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Publication No.: US20180073050A1Publication Date: 2018-03-15
- Inventor: Viktor Marius BOER , Nicolette Jasmijn Broers , Adam G. Lawrence
- Applicant: DSM IP ASSETS B.V
- Main IPC: C12P19/56
- IPC: C12P19/56 ; A23L2/60 ; C07H15/256 ; A23L27/30 ; A23K20/105 ; C12P15/00 ; C12N15/52 ; C12N9/90 ; C12N9/88 ; C12N9/10 ; C12N9/02

Abstract:
The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.
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