- 专利标题： METHODS AND COMPOSITIONS FOR ENZYME-MEDIATED SITE-SPECIFIC RADIOLABELING OF GLYCOPROTEINS
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申请号： US15826593申请日： 2017-11-29
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公开(公告)号： US20180078662A1公开(公告)日： 2018-03-22
- 发明人: Brian AGNEW , Robert AGGELER , Hee Chol KANG , Aimei CHEN , Kyle GEE
- 申请人： LIFE TECHNOLOGIES CORPORATION
- 主分类号： A61K51/10
- IPC分类号： A61K51/10 ; G01N33/534 ; C12P21/00 ; C07K16/18 ; G01N33/68 ; C12P21/06 ; C07K14/435 ; G01N33/58
摘要：
Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.