- 专利标题: ROTARY PLATFORM FOR CELL LYSING AND PURIFICATION AND METHOD OF USE
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申请号: US17818144申请日: 2022-08-08
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公开(公告)号: US20220379300A1公开(公告)日: 2022-12-01
- 发明人: Jason Bryant , Eric Yeaton , Dan Harris , Josiah Hackendorf , Steven Allen , Mark Talmer
- 申请人: Siemens Healthcare Diagnostics Inc.
- 申请人地址: US NY Tarrytown
- 专利权人: Siemens Healthcare Diagnostics Inc.
- 当前专利权人: Siemens Healthcare Diagnostics Inc.
- 当前专利权人地址: US NY Tarrytown
- 主分类号: B01L3/00
- IPC分类号: B01L3/00 ; C12N1/06 ; G01N35/00
摘要:
Magnetic beads having cell components of interest are translated between a sequence of processing wells in a tray without need for pipetting. The circular tray contains one or more sequences of wells each interconnected by a respective channel. The tray is rotated about a central axis and a magnet, an agitator, and a heater provided external to the tray enable magnetic bead translation, mixing, and incubation, respectively. The magnet proximate a well forms a cluster of beads. Manipulation of the tray in rotation and elevation results in translation of the cluster from one well, through a channel, and into an adjacent well. The well containing a cluster may be rotationally positioned in front of the agitator, the agitator extended into contact with the well, followed by mechanical agitation. The heater, disposed beneath the tray, may accept a well lowered thereto for selective heating.
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