发明授权
- 专利标题： Reagent and method for determination of lactate dehydrogenase
- 专利标题（中）： 用于确定乳酸脱氢酶的试剂和方法
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申请号： US3791931D申请日： 1971-11-30
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公开(公告)号： US3791931A公开(公告)日： 1974-02-12
- 发明人: BERGMEYER H , MOLLERING H , WEIMANN G , NAHER G , GRUBER W , THUM W , WAHLEFELD A
- 申请人： BOEHRINGER MANNHEIM GMBH
- 专利权人： Boehringer Mannheim Gmbh
- 当前专利权人： Boehringer Mannheim Gmbh
- 优先权： DE2061984 1970-12-16
- 主分类号： C12Q1/26
- IPC分类号： C12Q1/26 ; C12Q1/32 ; G01N31/14
摘要：
Lactate dehydrogenase is quickly and reliably determined by means of a color test by contacting the test composition with the reagent comprising serum albumin, pig''s heart diaphorase and nicotinamide-adenine-dinucleotide, wherein the diaphorase is preferably obtained from the protein fraction of pig''s heart insoluble in 1.6 to 2.8M ammonium sulphate by treatment with 0.1 to 0.3 percent weight/volume polyethyleneimine, heating at 70* to 80* C., adsorption on a weakly acidic cation exchanger and subsequent elution.
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