A process for separating porphyrins, which comprises(1) adjusting the pH of the liquid phase of a culture broth containing at least two porphyrins to about 2.5 to about 4 and collecting the resulting solid containing the porphyrins,(2) either (a) preparing an acidic aqueous solution of said solid and adjusting the pH of its liquid phase left after removal of insoluble matter to about 6 to about 8 to form an aqueous solution containing the porphyrins, or (b) preparing an acid-containing lower alcohol solution of said solid, adjusting the pH of its liquid phase left after removal of insoluble matter, adding water in an amount at least twice the amount of the solution to precipitate lower alkyl esters of the porphyrins, collecting the precipitates and dissolving them in an organic solvent to form an organic solvent solution of the porphyrin esters,(3) contacting the solution obtained in (a) or (b) in step (2) with a copolymer having a surface area of at least about 700 m.sup.2 /g and derived from(i) divinylbenzene,(ii) styrene or its functional derivative,(iii) an alkenyl ester of a benzenepolycarboxylic acid represented by the following formula ##STR1## wherein R represents an alkenyl group having 3 to 10 carbon atoms, and n is 2 or 3,thereby permitting adsorption of the porphyrins or their esters to the copolymer, and(4) thereafter, eluting the copolymer to obtain fractions of the individual porphyrins or their esters; and an adsorbent for use in the above-mentioned process.