A packing material for chromatography comprising a carrier having silanol groups on its surface, wherein Si in the silanol groups forms a Si--O--Si bond together with Si in a silyl group of the following formula I and Si in a silyl group of the following formula II, and the molar ratio of the silyl group of the formula II is from 0 to 2/3 based on the total amount of silyl groups: ##STR1## wherein each of R.sub.1 and R.sub.2 is a hydroxyl group, a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or an oxygen atom bonded to Si in a silanol group on the surface of the carrier, R.sub.3 is a 3-carbonyloxypropyl group, a 1-phenyleneethyl group or a 2-phenyleneethyl group, R.sub.4 is a hydrogen atom or a methyl group, and each of m and n is an integer of at least 1; and ##STR2## wherein each of R.sup.1 and R.sup.2 is a hydroxyl group, a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or an oxygen atom bonded to Si in a silanol group on the surface of the carrier, R.sub.5 is a methyl group, an ethyl group or a 3-glyceryloxypropyl group.