发明授权
US4904598A Novel plasmid shuttle vectors conferring spiramycin resistance in
streptomyces
失效
新型质粒穿梭载体赋予链霉菌中的螺旋霉素抗性
- 专利标题： Novel plasmid shuttle vectors conferring spiramycin resistance in streptomyces
- 专利标题（中）： 新型质粒穿梭载体赋予链霉菌中的螺旋霉素抗性
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申请号： US841981申请日： 1986-03-20
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公开(公告)号： US4904598A公开(公告)日： 1990-02-27
- 发明人: R. Nagaraja Rao
- 申请人： R. Nagaraja Rao
- 申请人地址： IN Indianapolis
- 专利权人： Eli Lilly and Company
- 当前专利权人： Eli Lilly and Company
- 当前专利权人地址： IN Indianapolis
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/20 ; C12N1/21 ; C12N15/00 ; C12N15/65 ; C12N15/76 ; C12R1/19 ; C12R1/465
摘要：
A novel gene conferring resistance to spiramycin in Streptomyces griseofuscus was cloned from a genomic library of Streptomyces ambofaciens DNA. An eighteen kb fragment of S. ambofaciens DNA surrounding the spiramycin resistance gene was isolated from this library on a plasmid cosmid designated pKC514. The novel spiramycin-resistance gene can be isolated on an .about.3.4 kb Sau3AI fragment by subcloning restriction fragments obtained from the pKC514 insert DNA. This Sau3AI fragment contains all of the information required for the expression of the spiramycin resistant phenotype in Streptomyces. Vectors and transformants containing the novel spiramycin resistance gene are provided.
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