发明授权
US5107422A Method and apparatus for measuring multiple optical properties of
biological specimens
失效
用于测量生物样本的多种光学性质的方法和装置
- 专利标题: Method and apparatus for measuring multiple optical properties of biological specimens
- 专利标题(中): 用于测量生物样本的多种光学性质的方法和装置
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申请号: US583075申请日: 1990-09-14
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公开(公告)号: US5107422A公开(公告)日: 1992-04-21
- 发明人: Louis A. Kamentsky , Lee D. Kamentsky
- 申请人: Louis A. Kamentsky , Lee D. Kamentsky
- 专利权人: Kamentsky Louis A,Kamentsky Lee D
- 当前专利权人: Kamentsky Louis A,Kamentsky Lee D
- 主分类号: G01N33/49
- IPC分类号: G01N33/49 ; G01N15/14 ; G01N33/53 ; G06K9/00
摘要:
The invention relates to an apparatus and methods for generating multiparameter optical data that characterize a population of cells. The invention includes the steps of scanning the cell population with a beam to produce sets of digital data samples, each sample set of digital data representing multiparameter optical interactions from a specific location within the cell population; storing the digital data, e.g., in a computer memory; locating a cell within the population, e.g., by comparing the digital data to a preselected threshold value; defining a neighborhood around the digital data representing the located cell; estimating a background level for the neighborhood based upon digital data corresponding to locations outside the neighborhood; and correcting each of the samples corresponding to the neighborhood with the estimated neighborhood background level to generate the optical data. The invention further relates to specific methods of background correction and data calibration as well as specific sampling features to enable precise estimates of multiple cellular constituents or other cell properties at high rates of speed.
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