发明授权
US5132223A Process and medium for cloning and long-term serial cultivation of adult
human endothelial cells
失效
用于克隆和长期连续培养成年人内皮细胞的方法和培养基
- 专利标题： Process and medium for cloning and long-term serial cultivation of adult human endothelial cells
- 专利标题（中）： 用于克隆和长期连续培养成年人内皮细胞的方法和培养基
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申请号： US609193申请日： 1990-11-05
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公开(公告)号： US5132223A公开(公告)日： 1992-07-21
- 发明人: Elliot M. Levine , Sandor S. Shapiro , Bruce E. Jarrell
- 申请人： Elliot M. Levine , Sandor S. Shapiro , Bruce E. Jarrell
- 申请人地址： PA Philadelphia
- 专利权人： Thomas Jefferson University
- 当前专利权人： Thomas Jefferson University
- 当前专利权人地址： PA Philadelphia
- 主分类号： C12N5/071
- IPC分类号： C12N5/071
摘要：
Vigorous growth of endothelial cells from human blood vessels in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.
公开/授权文献
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