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1.发明授权Process and medium for cloning and long-term serial cultivation of adult human endothelial cells 失效用于克隆和长期连续培养成年人内皮细胞的方法和培养基
公开(公告)号：US5132223A
公开(公告)日：1992-07-21
申请号：US609193
申请日：1990-11-05
IPC分类号： C12N5/071
CPC分类号： C12N5/069 , C12N2501/90 , C12N2501/91
摘要： Vigorous growth of endothelial cells from human blood vessels in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.
摘要翻译： 使用含有内皮细胞生长因子和肝素和/或硫酸葡聚糖的培养基实现体外人血管内皮细胞的生长旺盛。 获得13〜21个小时的细胞群繁殖时间为60〜85个群体倍增，克隆的人内皮细胞株具有相似的增殖能力。
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2.发明授权Process and medium for cloning and long-term serial cultivation of human endothelial cells 失效人类内皮细胞的克隆和长期连续培养的方法和培养基
公开(公告)号：US4994387A
公开(公告)日：1991-02-19
申请号：US210218
申请日：1988-06-17
IPC分类号： C12N5/071
CPC分类号： C12N5/069 , C12N2501/90 , C12N2501/91 , Y10S435/948
摘要： Vigorous growth of endothelial cells from human umbilical vein endothelial cells in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.
摘要翻译： 使用含有内皮细胞生长因子和肝素和/或硫酸葡聚糖的培养基实现体外来自人脐静脉内皮细胞的内皮细胞的剧烈生长。 获得13〜21个小时的细胞群繁殖时间为60〜85个群体倍增，克隆的人内皮细胞株具有相似的增殖能力。
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