发明授权
- 专利标题： Process for reduction of the matrix effect in a fructosamine determination assay
- 专利标题（中）： 降低木糖胺测定法中基质效应的方法
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申请号： US064336申请日： 1987-06-19
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公开(公告)号： US5156947A公开(公告)日： 1992-10-20
- 发明人: Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
- 申请人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
- 申请人地址： DEX Mannheim
- 专利权人： Boehringer Mannheim GmbH
- 当前专利权人： Boehringer Mannheim GmbH
- 当前专利权人地址： DEX Mannheim
- 优先权： DEX3620817 19860621
- 主分类号： C12Q1/26
- IPC分类号： C12Q1/26 ; C12Q1/28 ; C12Q1/30 ; C12Q1/44 ; C12Q1/54 ; G01N33/66
摘要：
This invention teaches a process for reducing protein matrix effects in assays for serum fructosamine. Blood or blood derived samples are used, and one adds two reagents, one of which reduces interference caused by non-specific reducing substances, the other of which eliminates turbidity. Incubation follows, and then the pH of the sample is adjusted and color forming reagent is added. In one embodiment, the incubation time is only 1-15 minutes. In another embodiment, the first reagent contains peroxidase.
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