发明授权
- 专利标题： Assay method using NAD synthetase
- 专利标题（中）： 使用NAD合成酶的测定方法
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申请号： US481752申请日： 1990-02-15
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公开(公告)号： US5206146A公开(公告)日： 1993-04-27
- 发明人: Hideo Misaki , Hidehiko Ishikawa , Kazuo Matsuura
- 申请人： Hideo Misaki , Hidehiko Ishikawa , Kazuo Matsuura
- 申请人地址： JPX Osaka
- 专利权人： Asahi Kasei Kogyo Kabushiki Kaisha
- 当前专利权人： Asahi Kasei Kogyo Kabushiki Kaisha
- 当前专利权人地址： JPX Osaka
- 优先权： JPX58-71513 19830425
- 主分类号： C12N9/00
- IPC分类号： C12N9/00 ; C12Q1/00 ; C12Q1/25 ; C12Q1/48 ; C12Q1/50
摘要：
An assay method for a component in a specimen containing any one of ATP, deamide-NAD and an amide donor which comprises performing a main reaction which comprises incubating the specimen with NAD synthetase in the presence of ATP, deamide-NAD, an amide donor and Mg.sup.++ to generate NAD; performing a coenzyme cycling reaction by combining the oxidation-reduction reaction system with coenzyme NAD and the oxidation-reduction reaction system with coenzyme reduced NAD, and measuring a consumed or generated component in the cycling reaction. The NAD synthetase can be produced by culturing the microorganism Bacillus licheniformis B-0844 FERM P-6809, in a culture medium, and isolating the thus-produced NAD synthetase therefrom.
公开/授权文献
- US5720741A Venous reservoir bag assembly 公开/授权日：1998-02-24
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