发明授权
- 专利标题： Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
- 专利标题（中）： 用热稳定DNA聚合酶 - 高温逆转录逆转录
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申请号： US82182申请日： 1993-06-24
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公开(公告)号： US5310652A公开(公告)日： 1994-05-10
- 发明人: David H. Gelfand , Thomas W. Myers
- 申请人： David H. Gelfand , Thomas W. Myers
- 申请人地址： NJ Nutley
- 专利权人： Hoffman-La Roche Inc.
- 当前专利权人： Hoffman-La Roche Inc.
- 当前专利权人地址： NJ Nutley
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N15/70 ; C12P19/34 ; C12Q1/68 ; C12Q1/70 ; C07H5/04 ; C07H15/12
摘要：
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
公开/授权文献
- USD404731S Three axis, three button mouse 公开/授权日：1999-01-26
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