发明授权
- 专利标题： Process for the production and renaturation of recombinant, biologically active, eukaryotic alkaline phosphatase
- 专利标题（中）： 重组，生物活性的真核碱性磷酸酶的生产和复性的方法
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申请号： US100124申请日： 1993-07-30
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公开(公告)号： US5434067A公开(公告)日： 1995-07-18
- 发明人: Uwe Michaelis , Rainer Rudolph , Michael Jarsch , Erhard Kopetzki , Helmut Burtscher , Gether Schumacher
- 申请人： Uwe Michaelis , Rainer Rudolph , Michael Jarsch , Erhard Kopetzki , Helmut Burtscher , Gether Schumacher
- 申请人地址： DEX Mannheim-Waldhof
- 专利权人： Boehringer Mannheim GmbH
- 当前专利权人： Boehringer Mannheim GmbH
- 当前专利权人地址： DEX Mannheim-Waldhof
- 优先权： DEX4225427.2 19920731
- 主分类号： C07K1/107
- IPC分类号： C07K1/107 ; C12N9/16 ; C12N9/96 ; C12N15/09 ; C12N15/55 ; C12N15/70 ; C12R1/19 ; C12N9/12 ; C07K1/36 ; C12N15/54
摘要：
In a process for the production of recombinant, biologically active, eukaryotic alkaline phosphatase a DNA sequence coding for a eukaryotic alkaline phosphatase is expressed in a prokaryotic host cell and the expression product is obtained in an active form by cell lysis, solubilization under denaturing conditions and subsequent renaturation. In this process the renaturation step is carried out in the presence of one or several stabilizing agents.
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