发明授权
US5541083A Method for producing secretable glycosyltransferases and other golgi
processing enzymes
失效
生产可分泌糖基转移酶和其他高尔基体加工酶的方法
- 专利标题: Method for producing secretable glycosyltransferases and other golgi processing enzymes
- 专利标题(中): 生产可分泌糖基转移酶和其他高尔基体加工酶的方法
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申请号: US209604申请日: 1994-03-10
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公开(公告)号: US5541083A公开(公告)日: 1996-07-30
- 发明人: James C. Paulson , Eryn Ujita-Lee , Karen J. Colley , Beverly Adler , Jeffrey K. Browne , Jasminder Weinstein
- 申请人: James C. Paulson , Eryn Ujita-Lee , Karen J. Colley , Beverly Adler , Jeffrey K. Browne , Jasminder Weinstein
- 申请人地址: CA Oakland CA Thousand Oaks
- 专利权人: The Regents of the University of California,Amgen
- 当前专利权人: The Regents of the University of California,Amgen
- 当前专利权人地址: CA Oakland CA Thousand Oaks
- 主分类号: C12N9/10
- IPC分类号: C12N9/10 ; C12N15/54 ; C12P19/18 ; C12P19/26 ; C12N15/52 ; C12P1/00 ; C12P21/06
摘要:
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
公开/授权文献
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