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公开(公告)号：US20120322730A1
公开(公告)日：2012-12-20
申请号：US13589026
申请日：2012-08-17
IPC分类号： A61K38/18 , C07K14/505
CPC分类号： C07K14/505 , C07K2319/30 , C12N15/62
摘要： Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythopoietin analogs, methods of production of the analogs, and compositions comprising the analogs.
摘要翻译： 公开了增加和维持哺乳动物血细胞比容的方法，包括施用红血球生成素的高糖蛋白类似物。 类似物可以比等效摩尔量的重组人促红细胞生成素施用频率更低，以获得可比较的目标血细胞比容并治疗贫血。 或者，可以施用较低摩尔量的高糖基化类似物以获得可比较的目标血细胞比容并治疗贫血。 还公开了新的高糖基化红细胞生成素类似物，类似物的生产方法和包含类似物的组合物。
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公开(公告)号：US08268588B2
公开(公告)日：2012-09-18
申请号：US13168913
申请日：2011-06-24
IPC分类号： C12N15/00 , C12N5/00 , C12N1/00 , C07K14/505
CPC分类号： C07K14/505 , C07K2319/30 , C12N15/62
摘要： Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythopoietin analogs, methods of production of the analogs, and compositions comprising the analogs.
摘要翻译： 公开了增加和维持哺乳动物血细胞比容的方法，包括施用红血球生成素的高糖蛋白类似物。 类似物可以比等效摩尔量的重组人促红细胞生成素施用频率更低，以获得可比较的目标血细胞比容并治疗贫血。 或者，可以施用较低摩尔量的高糖基化类似物以获得可比较的目标血细胞比容并治疗贫血。 还公开了新的高糖基化红细胞生成素类似物，类似物的制备方法和包含类似物的组合物。
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公开(公告)号：US5776772A
公开(公告)日：1998-07-07
申请号：US593865
申请日：1996-01-30
申请人： James G. Paulson , Eryn Ujita-Lee , Karen J. Colley , Beverly Adler , Jeffrey K. Browne , Jasminder Weinstein
发明人： James G. Paulson , Eryn Ujita-Lee , Karen J. Colley , Beverly Adler , Jeffrey K. Browne , Jasminder Weinstein
CPC分类号： C12P19/18 , C12N9/1051 , C12P19/26
摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
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4.发明授权Method for producing secretable glycosyltransferases and other golgi processing enzymes 失效生产可分泌糖基转移酶和其他高尔基体加工酶的方法
公开(公告)号：US5541083A
公开(公告)日：1996-07-30
申请号：US209604
申请日：1994-03-10
申请人： James C. Paulson , Eryn Ujita-Lee , Karen J. Colley , Beverly Adler , Jeffrey K. Browne , Jasminder Weinstein
发明人： James C. Paulson , Eryn Ujita-Lee , Karen J. Colley , Beverly Adler , Jeffrey K. Browne , Jasminder Weinstein
CPC分类号： C12P19/18 , C12N9/1051 , C12P19/26
摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
摘要翻译： 一种用于遗传工程细胞产生可溶性和可分泌的高尔基体加工酶而不是天然存在的膜结合酶的方法。 细胞被遗传工程化以表达缺少膜锚定和保留信号的糖基转移酶。 所得到的改变的酶变得可溶并且可被细胞分泌而不失去其催化活性。 由细胞分泌可溶性糖基转移酶提供增加的糖基转移酶的产生和简化的恢复。
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公开(公告)号：US5118627A
公开(公告)日：1992-06-02
申请号：US584132
申请日：1984-02-27
申请人： Jeffrey K. Browne
发明人： Jeffrey K. Browne
摘要： A microbial shuttle vector is disclosed which is independently replicative in bacterial cells and mammalian cells and includes in its DNA sequence bacterial plasmid sequences allowing selection and replication in bacterial cells, an SV40 viral origin of replication, and either an SV40 functional "early gene" promoter and functional "early gene" terminator or an SV40 functional "late gene" promoter and functional "late gene" terminator, the vector having a unique restriction endonuclease enzyme recognition site between the promoter and terminator for insertion of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional "late gene" into the "early gene" promoter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional "late gene" into the "late gene" promoter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector.
摘要翻译： 公开了在细菌细胞和哺乳动物细胞中独立地复制的微生物穿梭载体，并且在其DNA序列中包括允许在细菌细胞中选择和复制的细菌质粒序列，SV40病毒复制起点和SV40功能“早期基因”启动子 和功能“早期基因”终止子或SV40功能“晚期基因”启动子和功能“晚基因”终止子，该载体在用于插入外源基因的启动子和终止子之间具有独特的限制性内切核酸酶识别位点。 限制性内切核酸酶识别位点的存在促进将病毒功能“晚期基因”插入“早期基因”启动子/终止子载体中，允许穿梭载体转化成外源基因的裂解载体。 限制性内切核酸酶识别位点促进将病毒功能“晚期基因”插入到“晚期基因”启动子/终止子载体中的步骤允许穿梭载体转化成裂解载体。
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公开(公告)号：US20090017137A1
公开(公告)日：2009-01-15
申请号：US11977845
申请日：2007-10-26
CPC分类号： C07K14/505 , C07K2319/30 , C12N15/62
摘要： Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythropoietin analogs, methods of production of the analogs, and compositions comprising the analogs.
摘要翻译： 公开了增加和维持哺乳动物血细胞比容的方法，包括施用红血球生成素的高糖蛋白类似物。 类似物可以比等效摩尔量的重组人促红细胞生成素施用频率更低，以获得可比较的目标血细胞比容并治疗贫血。 或者，可以施用较低摩尔量的高糖基化类似物以获得可比较的目标血细胞比容并治疗贫血。 还公开了新的高糖基化促红细胞生成素类似物，类似物的生产方法和包含类似物的组合物。
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7.发明授权Method for producing secretable glycosyltransferases and other Golgi processing enzymes 失效生产可分泌糖基转移酶和其他高尔基体加工酶的方法
公开(公告)号：US5032519A
公开(公告)日：1991-07-16
申请号：US426577
申请日：1989-10-24
CPC分类号： C12N9/1051 , C12P19/18 , C12P19/26
摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a rThis invention was made with government support under Grant Contract Nos. GM-27904 and GM-11557 awarded by the National Institute of Health. The government has certain rights in this invention. The publications and other reference materials referred to herein to describe the background of the invention and to provide additional detail regarding its practice are hereby incorporated by reference. For convenience, the reference materials are numerically referenced and grouped in the appended bibliography.
摘要翻译： 一种用于遗传工程细胞产生可溶性和可分泌的高尔基体加工酶而不是天然存在的膜结合酶的方法。 细胞被遗传工程化以表达缺少膜锚定和保留信号的糖基转移酶。 所得到的改变的酶变得可溶并且可被细胞分泌而不失去其催化活性。 由细胞分泌可溶性糖基转移酶提供增加的糖基转移酶的产生和简化的恢复。
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公开(公告)号：US07973009B2
公开(公告)日：2011-07-05
申请号：US11977845
申请日：2007-10-26
IPC分类号： A61K38/18 , C07K14/505
CPC分类号： C07K14/505 , C07K2319/30 , C12N15/62
摘要： Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythropoietin analogs, methods of production of the analogs, and compositions comprising the analogs.
摘要翻译： 公开了增加和维持哺乳动物血细胞比容的方法，包括施用红血球生成素的高糖蛋白类似物。 类似物可以比等效摩尔量的重组人促红细胞生成素施用频率更低，以获得可比较的目标血细胞比容并治疗贫血。 或者，可以施用较低摩尔量的高糖基化类似物以获得可比较的目标血细胞比容并治疗贫血。 还公开了新的高糖基化促红细胞生成素类似物，类似物的生产方法和包含类似物的组合物。
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公开(公告)号：US20090023636A1
公开(公告)日：2009-01-22
申请号：US11977851
申请日：2007-10-26
IPC分类号： C07K14/505 , A61K38/18 , A61P7/06
CPC分类号： C07K14/505 , C07K2319/30 , C12N15/62
摘要： Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythopoietin analogs, methods of production of the analogs, and compositions comprising the analogs.
摘要翻译： 公开了增加和维持哺乳动物血细胞比容的方法，包括施用红血球生成素的高糖蛋白类似物。 类似物可以比等效摩尔量的重组人促红细胞生成素施用频率更低，以获得可比较的目标血细胞比容并治疗贫血。 或者，可以施用较低摩尔量的高糖基化类似物以获得可比较的目标血细胞比容并治疗贫血。 还公开了新的高糖基化红细胞生成素类似物，类似物的生产方法和包含类似物的组合物。
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公开(公告)号：US07304150B1
公开(公告)日：2007-12-04
申请号：US09723955
申请日：2000-11-27
IPC分类号： C07K14/505 , C07K16/46 , C07H21/00
CPC分类号： C07K14/505 , C07K2319/30 , C12N15/62
摘要： Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythopoietin analogs, methods of production of the analogs, and compositions comprising the analogs.
摘要翻译： 公开了增加和维持哺乳动物血细胞比容的方法，包括施用红血球生成素的高糖蛋白类似物。 类似物可以比等效摩尔量的重组人促红细胞生成素施用频率更低，以获得可比较的目标血细胞比容并治疗贫血。 或者，可以施用较低摩尔量的高糖基化类似物以获得可比较的目标血细胞比容并治疗贫血。 还公开了新的高糖基化红细胞生成素类似物，类似物的生产方法和包含类似物的组合物。
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