发明授权
- 专利标题: Phosphinothricin-resistance gene, and its use
- 专利标题(中): 氯氰菊酯抗性基因及其用途
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申请号: US487695申请日: 1995-06-07
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公开(公告)号: US5637489A公开(公告)日: 1997-06-10
- 发明人: Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
- 申请人: Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
- 申请人地址: DEX Frankfurt am Main
- 专利权人: Hoechst Aktiengesellschaft
- 当前专利权人: Hoechst Aktiengesellschaft
- 当前专利权人地址: DEX Frankfurt am Main
- 优先权: DEX3628747.4 19860823; DEX3637307.9 19861103; DEX3642829.9 19861216; DEX3700313.5 19870108
- 主分类号: C12N9/10
- IPC分类号: C12N9/10 ; C12N15/64 ; C12N15/82 ; C12P41/00 ; C12N15/05 ; C12N15/00
摘要:
Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.
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