- 专利标题： Mutant of Geotrichum candidum which produces novel enzyme system to selectively hydrolyze triglycerides
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申请号： US458306申请日： 1995-06-02
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公开(公告)号： US5654181A公开(公告)日： 1997-08-05
- 发明人: Dean A. Oester , Allen L. Hall , Stephen J. Vesper
- 申请人： Dean A. Oester , Allen L. Hall , Stephen J. Vesper
- 申请人地址： PA Plymouth Meeting
- 专利权人： Henkel Corporation
- 当前专利权人： Henkel Corporation
- 当前专利权人地址： PA Plymouth Meeting
- 主分类号： C11C1/04
- IPC分类号： C11C1/04 ; C11C3/00 ; C11C3/02 ; C12N1/14 ; C12N9/20 ; C12P7/64 ; C12R1/645 ; C12S3/18 ; C12P7/62
摘要：
A mutant fungal strain, namely G. candidum NRRL Y-552, mutant known as Henkel 9-2-3-9 produces a lipase called "UNLipase" providing a selectivity of 25:1 for oleic acid over palmitic acid by the assay procedure employed. UNLipase has a temperature range of operation of between 0 and 40 degrees Celsius (and no activity over 55 degrees Celsius). The optimum pH ranges are between 7.5 and 8.5. Magnesium cations increase activity, whereas calcium cations are inhibitory. The molecular weight of the protein appears to be 65 kDa by size exclusion chromatography. UNLipase shows a high degree of selectivity for hydrolysis, esterification and transesterification.
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