发明授权
- 专利标题: Methods for harvesting adipose tissue containing autologous microvascular endothelial cells
- 专利标题(中): 收集含有自体微血管内皮细胞的脂肪组织的方法
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申请号: US647155申请日: 1996-05-09
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公开(公告)号: US5744360A公开(公告)日: 1998-04-28
- 发明人: Can B. Hu , Keith E. Myers , Robert C. Peterson
- 申请人: Can B. Hu , Keith E. Myers , Robert C. Peterson
- 申请人地址: IL Deerfield
- 专利权人: Baxter International Inc.
- 当前专利权人: Baxter International Inc.
- 当前专利权人地址: IL Deerfield
- 主分类号: A61B10/02
- IPC分类号: A61B10/02 ; A61F2/06 ; A61J1/00 ; A61J1/20 ; A61M1/00 ; A61M5/31 ; A61M5/315 ; A61M5/32 ; C12N5/00 ; A61B17/20 ; A61N1/30
摘要:
Disclosed herein are methods for harvesting adipose tissue so as to preserve an increased population of viable microvascular endothelial cells. Adipose tissue containing microvascular endothelial cells is harvested using a collection apparatus incorporating an elongate cannula having apertures with tissue cutting edges. A sub-ambient pressure is applied to a lumen in the cannula to draw the adipose tissue through the aperture where it is then severed using the cutting edge to disrupt the connective adipose matrix. This harvesting provides a cleaner, more homogeneous sample of adipose tissue, thereby increasing the population of viable microvascular endothelial cells obtained through further processing. Rapid and easy methods for the further homogenization of the harvested adipose tissue are also disclosed.
公开/授权文献
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