- 专利标题: Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
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申请号: US564955申请日: 1995-11-30
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公开(公告)号: US5811238A公开(公告)日: 1998-09-22
- 发明人: Willem P. C. Stemmer , Andreas Crameri
- 申请人: Willem P. C. Stemmer , Andreas Crameri
- 申请人地址: ANX De Ruyderkade
- 专利权人: Affymax Technologies N.V.
- 当前专利权人: Affymax Technologies N.V.
- 当前专利权人地址: ANX De Ruyderkade
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; A61K38/00 ; A61K39/395 ; C07K14/435 ; C07K14/545 ; C07K16/00 ; C12N9/38 ; C12N9/86 ; C12N15/00 ; C12N15/10 ; C12P21/08 ; C12Q1/68 ; C40B40/02 ; G01N33/53 ; G01N33/566
摘要:
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
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- USD330411S Lifter for a toilet seat 公开/授权日:1992-10-20
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